Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

In situ cryo electron tomography of cryo focused ion beam milled samples has emerged in
recent years as a powerful technique for structural studies of macromolecular complexes in …

Cryo-electron microscopy (cryo-EM) enables macromolecular structure determination in vitro
and inside cells. In addition to aligning individual particles, accurate registration of sample …

Cryoelectron tomography directly visualizes heterogeneous macromolecular structures in
their native and complex cellular environments. However, existing computer-assisted …

The recent proliferation of cryo-electron tomography (cryo-ET) techniques has led to the cryo-
ET resolution revolution. Meanwhile, significant efforts have been made to improve the …

Cryo‐electron tomography analysis involves the selection of macromolecular complexes to
be used for subsequent sub‐tomogram averaging and structure determination. Here, we …

Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …

The bacterial flagellar motor can rotate in counterclockwise (CCW) or clockwise (CW)
senses, and transitions are controlled by the phosphorylated form of the response regulator …

Tomographic reconstruction of cryopreserved specimens imaged in an electron microscope
followed by extraction and averaging of sub-volumes has been successfully used to derive …