Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Single-cell analysis of human mononuclear phagocytes reveals subset-defining markers and identifies circulating inflammatory dendritic cells

CA Dutertre, E Becht, SE Irac, A Khalilnezhad… - Immunity, 2019 - cell.com
Human mononuclear phagocytes comprise phenotypically and functionally overlapping
subsets of dendritic cells (DCs) and monocytes, but the extent of their heterogeneity and …

Gut CD4+ T cell phenotypes are a continuum molded by microbes, not by TH archetypes

E Kiner, E Willie, B Vijaykumar, K Chowdhary… - Nature …, 2021 - nature.com
Abstract CD4+ effector lymphocytes (Teff) are traditionally classified by the cytokines they
produce. To determine the states that Teff cells actually adopt in frontline tissues in vivo, we …

[HTML][HTML] Single-cell proteo-genomic reference maps of the hematopoietic system enable the purification and massive profiling of precisely defined cell states

S Triana, D Vonficht, L Jopp-Saile, S Raffel, R Lutz… - Nature …, 2021 - nature.com
Single-cell genomics technology has transformed our understanding of complex cellular
systems. However, excessive cost and a lack of strategies for the purification of newly …

[HTML][HTML] Non-terminally exhausted tumor-resident memory HBV-specific T cell responses correlate with relapse-free survival in hepatocellular carcinoma

Y Cheng, B Gunasegaran, HD Singh, CA Dutertre… - Immunity, 2021 - cell.com
Hepatocellular carcinoma (HCC) often develops following chronic hepatitis B virus (HBV)
infection and responds poorly to immune checkpoint blockade. Here, we examined the …

diffcyt: Differential discovery in high-dimensional cytometry via high-resolution clustering

LM Weber, M Nowicka, C Soneson… - Communications …, 2019 - nature.com
High-dimensional flow and mass cytometry allow cell types and states to be characterized in
great detail by measuring expression levels of more than 40 targeted protein markers per …

Advances in automated real‐time flow cytometry for monitoring of bioreactor processes

AL Heins, MD Hoang… - Engineering in life …, 2022 - Wiley Online Library
Flow cytometry and its technological possibilities have greatly advanced in the past decade
as analysis tool for single cell properties and population distributions of different cell types in …

Melanoma progression and prognostic models drawn from single-cell, spatial maps of benign and malignant tumors

NR Love, C Williams, EE Killingbeck, A Merleev… - Science …, 2024 - science.org
Melanoma clinical outcomes emerge from incompletely understood genetic mechanisms
operating within the tumor and its microenvironment. Here, we used single-cell RNA-based …

A cancer biologist's primer on machine learning applications in high‐dimensional cytometry

TJ Keyes, P Domizi, YC Lo, GP Nolan… - Cytometry Part …, 2020 - Wiley Online Library
The application of machine learning and artificial intelligence to high‐dimensional cytometry
data sets has increasingly become a staple of bioinformatic data analysis over the past …

Multimodal and spatially resolved profiling identifies distinct patterns of T cell infiltration in nodal B cell lymphoma entities

T Roider, MA Baertsch, D Fitzgerald, H Voehringer… - Nature Cell …, 2024 - nature.com
The redirection of T cells has emerged as an attractive therapeutic principle in B cell non-
Hodgkin lymphoma (B-NHL). However, a detailed characterization of lymphoma-infiltrating T …