The translation of novel discoveries from basic research to clinical application is a long,
often inefficient, and thus costly process. Accordingly, the process of drug development …

The CRISPR/Cas has been recently shown to be a powerful genome-editing tool in a variety
of organisms. However, these studies are mainly focused on protein-coding genes. The …

Sequence-specific nucleases (SSN s) have been used successfully in homology-directed
repair (HDR)-mediated gene targeting (GT) in many organisms. However, break-induced GT …

Gene tagging with fluorescent proteins is essential for investigations of the dynamic
properties of cellular proteins. CRISPR–Cas9 technology is a powerful tool for inserting …

CRISPR/Cas9, a bacterial adaptive immune system derived genome-editing technique, has
become to be one of the most compelling topics in biotechnology. Bombyx mori is an …

Transgenic animal technology is one of the fastest growing biotechnology areas. It is used to
integrate exogenous genes into the animal genome by genetic engineering technology so …

Kluyveromyces marxianus DMKU3‐1042 is a thermotolerant yeast strain suitable for high‐
temperature ethanol fermentation and genetic engineering with linear DNA. We have …

Gene targeting is a challenge in organisms where non-homologous end-joining is the
predominant form of recombination. We show that cell division cycle synchronization can be …

Evidence for the involvement of the nonhomologous end joining (NHEJ) pathway in
Agrobacterium‐mediated transferred DNA (T‐DNA) integration into the genome of the model …

Penicillium decumbens is an important industrial filamentous fungus and has been widely
used in biorefinery due to its high production of cellulase and hemicellulase. However …