Recent strategies for the immobilization of therapeutic enzymes
Therapeutic enzymes play important roles in modern medicine due to their high affinity and
specificity. However, it is very expensive to use them in clinical medicine because of their …
specificity. However, it is very expensive to use them in clinical medicine because of their …
Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis
Incorporating non-standard amino acids (NSAAs) into proteins enables new chemical
properties, new structures, and new functions. In recent years, improvements in cell-free …
properties, new structures, and new functions. In recent years, improvements in cell-free …
Designing an enzyme assembly line for green cascade processes using bio-orthogonal chemistry
Two non-canonical amino acids (ncAAs) with bio-orthogonal reactive groups, namely, p-
azido-L-phenylalanine (p-AzF) and p-propargyloxy-L-phenylalanine (p-PaF), were …
azido-L-phenylalanine (p-AzF) and p-propargyloxy-L-phenylalanine (p-PaF), were …
Enhanced enzyme stability through site-directed covalent immobilization
JCY Wu, CH Hutchings, MJ Lindsay, CJ Werner… - Journal of …, 2015 - Elsevier
Breakthroughs in enzyme immobilization have enabled increased enzyme recovery and
reusability, leading to significant decreases in the cost of enzyme use and fueling …
reusability, leading to significant decreases in the cost of enzyme use and fueling …
An emerging paradigm to develop analytical methods based on immobilized transmembrane proteins and its applications in drug discovery
The way to immobilize a protein onto a solid surface is the cornerstone for developing many
bioanalytical assays which are applied in protein functional assay, protein-ligand interaction …
bioanalytical assays which are applied in protein functional assay, protein-ligand interaction …
Non-canonical amino acid labeling in proteomics and biotechnology
Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique
bioorthogonal chemical groups during de novo synthesis by taking advantage of both …
bioorthogonal chemical groups during de novo synthesis by taking advantage of both …
Artificial metalloenzyme design with unnatural amino acids and non-native cofactors
Y Yu, C Hu, L Xia, J Wang - ACS Catalysis, 2018 - ACS Publications
There are 20 proteinogenic amino acids and a limited number of cofactors naturally
available to build enzymes. Genetic codon expansion enables us to incorporate more than …
available to build enzymes. Genetic codon expansion enables us to incorporate more than …
Lyophilized Escherichia Coli-Based Cell-Free Systems for Robust, High-Density, Long-Term Storage
Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant protein production
and engineering. One drawback of cell-free technology is the necessity to store the major …
and engineering. One drawback of cell-free technology is the necessity to store the major …
Investigating the effect of two-point surface attachment on enzyme stability and activity
Immobilization on solid supports provides an effective way to improve enzyme stability and
simplify downstream processing for biotechnological applications, which has been widely …
simplify downstream processing for biotechnological applications, which has been widely …
[HTML][HTML] The emerging age of cell-free synthetic biology
The engineering of and mastery over biological parts has catalyzed the emergence of
synthetic biology. This field has grown exponentially in the past decade. As increasingly …
synthetic biology. This field has grown exponentially in the past decade. As increasingly …