Hydrogels as functional components in artificial cell systems

ME Allen, JW Hindley, DK Baxani, O Ces… - Nature Reviews …, 2022 - nature.com
Recent years have seen substantial efforts aimed at constructing artificial cells from various
molecular components with the aim of mimicking the processes, behaviours and …

Recent Advances in Microfluidic Technologies for the Construction of Artificial Cells

S Tan, Y Ai, X Yin, Z Xue, X Fang… - Advanced Functional …, 2023 - Wiley Online Library
Artificial cells are synthetic constructs that emulate natural cells, with potential applications in
areas of energy science, environmental treatment, and the study of life's origins …

Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades

JC Breger, JN Vranish, E Oh, MH Stewart… - Nature …, 2023 - nature.com
Access to efficient enzymatic channeling is desired for improving all manner of designer
biocatalysis. We demonstrate that enzymes constituting a multistep cascade can self …

Biomimetic behaviors in hydrogel artificial cells through embedded organelles

ME Allen, JW Hindley, N O'Toole… - Proceedings of the …, 2023 - National Acad Sciences
Artificial cells are biomimetic structures formed from molecular building blocks that replicate
biological processes, behaviors, and architectures. Of these building blocks, hydrogels have …

Phospholipid synthesis inside phospholipid membrane vesicles

S Eto, R Matsumura, Y Shimane, M Fujimi… - Communications …, 2022 - nature.com
Construction of living artificial cells from genes and molecules can expand our
understanding of life system and establish a new aspect of bioengineering. However, growth …

Building programmable multicompartment artificial cells incorporating remotely activated protein channels using microfluidics and acoustic levitation

J Li, WD Jamieson, P Dimitriou, W Xu, P Rohde… - Nature …, 2022 - nature.com
Intracellular compartments are functional units that support the metabolism within living
cells, through spatiotemporal regulation of chemical reactions and biological processes …

Chemical Zymogens and Transmembrane Activation of Transcription in Synthetic Cells

DG Andersen, AB Pedersen, MH Jørgensen… - Advanced …, 2024 - Wiley Online Library
In this work, synthetic cells equipped with an artificial signaling pathway that connects an
extracellular trigger event to the activation of intracellular transcription are engineered …

Spatial confinement toward creating artificial living systems

L Shang, F Ye, M Li, Y Zhao - Chemical Society Reviews, 2022 - pubs.rsc.org
Lifeforms are regulated by many physicochemical factors, and these factors could be
controlled to play a role in the construction of artificial living systems. Among these factors …

Recent advances in cell membrane camouflage-based biosensing application

X Yu, L Sha, Q Liu, Y Zhao, H Fang, Y Cao… - Biosensors and …, 2021 - Elsevier
Cell membrane, a semi-permeable membrane composed of phospholipid bilayers, is a
natural barrier to prevent extracellular substances from freely entering the cell. Cell …

Continuous cell-free replication and evolution of artificial genomic DNA in a compartmentalized gene expression system

H Okauchi, N Ichihashi - ACS Synthetic Biology, 2021 - ACS Publications
In all living organisms, genomic DNA continuously replicates by the proteins encoded in
itself and undergoes evolution through many generations of replication. This continuous …