The polymerase chain reaction: miracle or mirage? A critical review of its uses and limitations in diagnosis and research
PA Wright, D Wynford‐Thomas - The Journal of Pathology, 1990 - Wiley Online Library
Since publication of the polymerase chain reaction (PCR) technique in 1985 (Saiki et al.
Science 1985; 230 1350-1 354), there has been an explosion of reports on its use in …
Science 1985; 230 1350-1 354), there has been an explosion of reports on its use in …
Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
KH Wilson, RB Blitchington… - Journal of clinical …, 1990 - Am Soc Microbiol
The sequence of small-subunit rRNA varies in an orderly manner across phylogenetic lines
and contains segments that are conserved at the species, genus, or kingdom level. By …
and contains segments that are conserved at the species, genus, or kingdom level. By …
[图书][B] Ribosomal RNA analysis of microorganisms as they occur in nature
DM Ward, MM Bateson, R Weller, AL Ruff-Roberts - 1992 - Springer
Advances in molecular biology are now providing the means for solving long-standing
problems in microbiology. One of the best examples is the development of a rational …
problems in microbiology. One of the best examples is the development of a rational …
Potential use of PCR-amplified ribosomal intergenic sequences in the detection and differentiation of Verticillium wilt pathogens
RN Nazar, X Hu, J Schmidt, D Culham… - … and Molecular Plant …, 1991 - Elsevier
Verticillium wilt pathogens are widespread throughout the world and an important factor in
most agricultural economies. The ribosomal genes from the two major pathogenic species …
most agricultural economies. The ribosomal genes from the two major pathogenic species …
[图书][B] Molecular detection of human bacterial pathogens
D Liu - 2011 - books.google.com
As more original molecular protocols and subsequent modifications are described in the
literature, it has become difficult for those not directly involved in the development of these …
literature, it has become difficult for those not directly involved in the development of these …
Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens
A Meier, DH Persing, M Finken… - Journal of Clinical …, 1993 - Am Soc Microbiol
Analysis based on comparisons of 16S rRNA sequences provides a rapid and reliable
approach to identifying human pathogens. By directing oligonucleotide primers at …
approach to identifying human pathogens. By directing oligonucleotide primers at …
Rocky Mountain “spotless” and “almost spotless” fever: a wolf in sheep's clothing
Abstract In 10 (10.8%) of 93 laboratory-confirmed or probable cases of Rocky Mountain
spotted fever seen at Duke University Medical Center from 1969 to 1991, illness without rash …
spotted fever seen at Duke University Medical Center from 1969 to 1991, illness without rash …
The polymerase chain reaction: clinical applications
TJ White, R Madej, DH Persing - Advances in clinical chemistry, 1992 - Elsevier
Publisher Summary This chapter discusses the clinical applications of polymerase chain
reaction (PCR). The chapter identifies those diseases for which the PCR offers an …
reaction (PCR). The chapter identifies those diseases for which the PCR offers an …
Efficacy of doxycycline, azithromycin, or trovafloxacin for treatment of experimental Rocky Mountain spotted fever in dogs
EB Breitschwerdt, MG Papich, BC Hegarty… - Antimicrobial agents …, 1999 - Am Soc Microbiol
Dogs were experimentally inoculated with Rickettsia rickettsii (canine origin) in order to
compare the efficacies of azithromycin and trovafloxacin to that of the current antibiotic …
compare the efficacies of azithromycin and trovafloxacin to that of the current antibiotic …