Cryo-soft X-ray tomography: using soft X-rays to explore the ultrastructure of whole cells

M Harkiolaki, MC Darrow, MC Spink… - Emerging topics in …, 2018 - portlandpress.com
Cryo-soft X-ray tomography is an imaging technique that addresses the need for mesoscale
imaging of cellular ultrastructure of relatively thick samples without the need for staining or …

[HTML][HTML] A modular platform for automated cryo-FIB workflows

S Klumpe, HKH Fung, SK Goetz, I Zagoriy, B Hampoelz… - Elife, 2021 - ncbi.nlm.nih.gov
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB)
has matured into a preparation method widely used for cellular cryo-electron tomography …

Optimising complementary soft tissue synchrotron X-ray microtomography for reversibly-stained central nervous system samples

MC Strotton, AJ Bodey, K Wanelik, MC Darrow… - Scientific reports, 2018 - nature.com
Synchrotron radiation microtomography (SRμCT) is a nominally non-destructive 3D imaging
technique which can visualise the internal structures of whole soft tissues. As a multi-stage …

SuRVoS 2: accelerating annotation and segmentation for large volumetric bioimage workflows across modalities and scales

A Pennington, ONF King, WM Tun, EML Ho… - Frontiers in Cell and …, 2022 - frontiersin.org
As sample preparation and imaging techniques have expanded and improved to include a
variety of options for larger sized and numbers of samples, the bottleneck in volumetric …

Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare events

CJ Guérin, A Kremer, P Borghgraef, AY Shih… - Methods in cell …, 2019 - Elsevier
Volume electron microscopy allows for the automated acquisition of serial-section imaging
data that can be reconstructed in three-dimensions (3D) to provide a detailed, geometrically …

Okapi-EM: A napari plugin for processing and analyzing cryogenic serial focused ion beam/scanning electron microscopy images

LMA Perdigão, EML Ho, ZC Cheng, NBY Yee… - Biological …, 2023 - cambridge.org
An emergent volume electron microscopy technique called cryogenic serial plasma focused
ion beam milling scanning electron microscopy (pFIB/SEM) can decipher complex biological …

[HTML][HTML] From Voxels to Viruses: Using Deep Learning and Crowdsourcing to Understand a Virus Factory

A Pennington, ONF King… - Citizen …, 2024 - theoryandpractice …
Many bioimaging research projects require objects of interest to be identified, located, and
then traced to allow quantitative measurement. Depending on the complexity of the system …

Computing for optimized biological microscopy data processing and analysis at The Rosalind Franklin Institute

LMA Perdigão, EML Ho, AH Pennington… - Microscopy and …, 2022 - cambridge.org
Recent developments in experimental microscopy techniques have led to improvements in
the way we visualize various biological phenomena. Presently, state-of-art microscopy …

Okapi-EM–a napari plugin for processing and analysing cryogenic serial FIB/SEM images

LMA Perdigão, EML Ho, ZC Cheng, NB Yee, T Glen… - bioRxiv, 2022 - biorxiv.org
An emergent volume electron microscopy (vEM) technique called cryogenic serial plasma
focused ion beam milling scanning electron microscopy (pFIB/SEM) can decipher complex …