Super-resolution microscopy demystified

L Schermelleh, A Ferrand, T Huser, C Eggeling… - Nature cell …, 2019 - nature.com
Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Super-resolution microscopy: shedding light on the cellular plasma membrane

MB Stone, SA Shelby, SL Veatch - Chemical reviews, 2017 - ACS Publications
Lipids and the membranes they form are fundamental building blocks of cellular life, and
their geometry and chemical properties distinguish membranes from other cellular …

Fixation can change the appearance of phase separation in living cells

S Irgen-Gioro, S Yoshida, V Walling, S Chong - elife, 2022 - elifesciences.org
Fixing cells with paraformaldehyde (PFA) is an essential step in numerous biological
techniques as it is thought to preserve a snapshot of biomolecular transactions in living cells …

iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy

V Louvel, R Haase, O Mercey, MH Laporte… - Nature …, 2023 - nature.com
Expansion microscopy (ExM) is a highly effective technique for super-resolution
fluorescence microscopy that enables imaging of biological samples beyond the diffraction …

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)

MH Laporte, N Klena, V Hamel, P Guichard - Nature methods, 2022 - nature.com
Cryofixation has proven to be the gold standard for efficient preservation of native cell
ultrastructure compared to chemical fixation, but this approach is not widely used in …

Hyaluronan in the lymphatics: The key role of the hyaluronan receptor LYVE-1 in leucocyte trafficking

DG Jackson - Matrix Biology, 2019 - Elsevier
LYVE-1, a close relative of the leucocyte receptor, CD44, is the main receptor for hyaluronan
(HA) in lymphatic vessel endothelium and a widely used marker for distinguishing between …

Electrochemiluminescence loss in photobleaching

D Han, B Goudeau, D Manojlovic, D Jiang… - Angewandte …, 2021 - Wiley Online Library
The effects of photobleaching on electrochemiluminescence (ECL) was investigated for the
first time. The plasma membrane of Chinese Hamster Ovary (CHO) cells was labeled with a …

PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells

S Prokop, P Ábrányi-Balogh, B Barti, M Vámosi… - Nature …, 2021 - nature.com
Immunolabeling and autoradiography have traditionally been applied as the methods-of-
choice to visualize and collect molecular information about physiological and pathological …

Aptamers with tunable affinity enable single‐molecule tracking and localization of membrane receptors on living cancer cells

P Delcanale, D Porciani, S Pujals… - Angewandte Chemie …, 2020 - Wiley Online Library
Tumor cell‐surface markers are usually overexpressed or mutated protein receptors for
which spatiotemporal regulation differs between and within cancers. Single‐molecule …

Direct observation of the effects of chemical fixation in MNT-1 cells: A SE-ADM and Raman study

R Mastrangelo, T Okada, T Ogura… - Proceedings of the …, 2023 - National Acad Sciences
Aldehydes fixation was accidentally discovered in the early 20th century and soon became a
widely adopted practice in the histological field, due to an excellent staining enhancement in …