Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …

Reagents that bind tightly and specifically to biomolecules of interest remain essential in the
exploration of biology and in their ultimate application to medicine. Besides ligands for …

Asparaginyl endopeptidases (AEP) are cysteine proteases found in mammalian and plant
cells. Several AEP isoforms from plant species were found to exhibit transpeptidase activity …

Overexpressed Mdm2 and its 7homolog MdmX impair p53 activity in many cancers. Small
molecules mimicking a p53 peptide can effectively inhibit Mdm2 but not MdmX. Here, we …

Butelase-1, an asparaginyl endopeptidase or legumain, is the prototypical and fastest
known Asn/Asp-specific peptide ligase. It is highly useful for engineering and …

Transpeptidase‐catalyzed protein and peptide modifications have been widely utilized for
generating conjugates of interest for biological investigation or therapeutic applications …

Asparaginyl endopeptidases (AEPs) are cysteine proteases that control a myriad of cellular
functions in plants, including maturation of seed storage proteins and programmed cell …

Butelase-1, the fastest known Asn/Asp-specific peptide ligase capable of catalyzing peptide
ligation and cyclization, holds promising application prospects in the fields of food and …

Enzyme‐catalysed site‐specific protein modifications enable the precision manufacture of
conjugates for the study of protein function and/or for therapeutic or diagnostic applications …

Making peptide bonds is tightly controlled by genetic code and machinery which includes
cofactors, ATP, and RNAs. In this regard, the stand-alone and genetic-code-independent …