CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Compendium of human transcription factor effector domains
Transcription factors (TFs) regulate gene expression by binding to DNA sequences and
modulating transcriptional activity through their effector domains. Despite the central role of …
modulating transcriptional activity through their effector domains. Despite the central role of …
Transcription factors interact with RNA to regulate genes
O Oksuz, JE Henninger, R Warneford-Thomson… - Molecular cell, 2023 - cell.com
Transcription factors (TFs) orchestrate the gene expression programs that define each cell's
identity. The canonical TF accomplishes this with two domains, one that binds specific DNA …
identity. The canonical TF accomplishes this with two domains, one that binds specific DNA …
Large-scale mapping and mutagenesis of human transcriptional effector domains
Human gene expression is regulated by more than 2,000 transcription factors and chromatin
regulators,. Effector domains within these proteins can activate or repress transcription …
regulators,. Effector domains within these proteins can activate or repress transcription …
An activity-specificity trade-off encoded in human transcription factors
Transcription factors (TFs) control specificity and activity of gene transcription, but whether a
relationship between these two features exists is unclear. Here we provide evidence for an …
relationship between these two features exists is unclear. Here we provide evidence for an …
Simple biochemical features underlie transcriptional activation domain diversity and dynamic, fuzzy binding to Mediator
AL Sanborn, BT Yeh, JT Feigerle, CV Hao… - Elife, 2021 - elifesciences.org
Gene activator proteins comprise distinct DNA-binding and transcriptional activation
domains (ADs). Because few ADs have been described, we tested domains tiling all yeast …
domains (ADs). Because few ADs have been described, we tested domains tiling all yeast …
Identification and functional characterization of transcriptional activators in human cells
Transcription is orchestrated by thousands of transcription factors (TFs) and chromatin-
associated proteins, but how these are causally connected to transcriptional activation is …
associated proteins, but how these are causally connected to transcriptional activation is …
High-throughput discovery and characterization of human transcriptional effectors
Thousands of proteins localize to the nucleus; however, it remains unclear which contain
transcriptional effectors. Here, we develop HT-recruit, a pooled assay where protein libraries …
transcriptional effectors. Here, we develop HT-recruit, a pooled assay where protein libraries …
Multifunctional intrinsically disordered regions in transcription factors
M Már, K Nitsenko… - Chemistry–A European …, 2023 - Wiley Online Library
Eukaryotic transcription factors (TFs) are the final integrators of a complex molecular
feedback mechanism that interfaces with the genome, consolidating information for …
feedback mechanism that interfaces with the genome, consolidating information for …
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts
Transcriptional effectors are protein domains known to activate or repress gene expression;
however, a systematic understanding of which effector domains regulate transcription across …
however, a systematic understanding of which effector domains regulate transcription across …