Technical challenges of working with extracellular vesicles

MI Ramirez, MG Amorim, C Gadelha, I Milic, JA Welsh… - Nanoscale, 2018 - pubs.rsc.org
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with
potential applications in diagnosis, prognosis and therapeutic guidance in most pathological …

Data normalization strategies for microRNA quantification

H Schwarzenbach, AM Da Silva, G Calin… - Clinical …, 2015 - academic.oup.com
BACKGROUND Different technologies, such as quantitative real-time PCR or microarrays,
have been developed to measure microRNA (miRNA) expression levels. Quantification of …

Standardization of sample collection, isolation and analysis methods in extracellular vesicle research

KW Witwer, EI Buzás, LT Bemis, A Bora… - Journal of …, 2013 - Taylor & Francis
The emergence of publications on extracellular RNA (exRNA) and extracellular vesicles
(EV) has highlighted the potential of these molecules and vehicles as biomarkers of disease …

MicroRNA: function, detection, and bioanalysis

H Dong, J Lei, L Ding, Y Wen, H Ju, X Zhang - Chemical reviews, 2013 - ACS Publications
Mature microRNAs (miRNAs) are a class of evolutionally conserved, single-stranded, small
(approximately 19− 23 nucleotides), endogenously expressed, and non-protein-coding …

Concise review: developing best-practice models for the therapeutic use of extracellular vesicles

AT Reiner, KW Witwer, BWM Van Balkom… - Stem cells …, 2017 - academic.oup.com
Growing interest in extracellular vesicles (EVs, including exosomes and microvesicles) as
therapeutic entities, particularly in stem cell-related approaches, has underlined the need for …

MicroRNA profiling: approaches and considerations

CC Pritchard, HH Cheng, M Tewari - Nature Reviews Genetics, 2012 - nature.com
MicroRNAs (miRNAs) are small RNAs that post-transcriptionally regulate the expression of
thousands of genes in a broad range of organisms in both normal physiological contexts …

Circulating microRNA biomarker studies: pitfalls and potential solutions

KW Witwer - Clinical chemistry, 2015 - academic.oup.com
BACKGROUND Circulating microRNAs have been proposed as disease biomarkers that
may aid in risk assessment, diagnosis, prognosis, and monitoring of treatment response …

Circulating miRNA analysis for cancer diagnostics and therapy

L Valihrach, P Androvic, M Kubista - Molecular Aspects of Medicine, 2020 - Elsevier
Successful treatment of cancer depends on early diagnosis and effective monitoring of
patients' response to therapy. Traditional tools based on tumor biopsies lack the sensitivity …

MicroRNAs in heart failure: from biomarker to target for therapy

EL Vegter, P van der Meer, LJ de Windt… - European journal of …, 2016 - Wiley Online Library
MicroRNAs (miRNAs) are increasingly recognized to play important roles in cardiovascular
diseases, including heart failure. These small, non‐coding RNAs have been identified in …

Normalization of circulating microRNA expression data obtained by quantitative real-time RT-PCR

F Marabita, P De Candia, A Torri… - Briefings in …, 2016 - academic.oup.com
The high-throughput analysis of microRNAs (miRNAs) circulating within the blood of healthy
and diseased individuals is an active area of biomarker research. Whereas quantitative real …