In recent years, chemical crosslinking of protein complexes and the identification of
crosslinked residues by mass spectrometry (XL-MS; sometimes abbreviated as CX-MS) has …

The separation of sister chromatids during anaphase is the culmination of mitosis and one of
the most strikingly beautiful examples of cellular movement. It consists of two distinct …

The spindle assembly checkpoint (SAC) is a unique signalling mechanism that responds to
the state of attachment of the kinetochore to spindle microtubules. SAC signalling is …

The conserved Ndc80 kinetochore complex, Ndc80c, is the principal link between mitotic
spindle microtubules and centromere-associated proteins. We used AlphaFold 2 (AF2) to …

Chromosome segregation requires sister kinetochores to attach microtubules emanating
from opposite spindle poles. Proper attachments come under tension and are stabilized, but …

Strong kinetochore-microtubule attachments are essential for faithful segregation of sister
chromatids during mitosis. The Dam1 and Ndc80 complexes are the main microtubule …

METHODS NDC80 gene expression was analyzed by real-time reverse transcription
polymerase chain reaction in 47 paired hepatocellular carcinoma (HCC) and adjacent …

The spindle assembly checkpoint (SAC) is a surveillance mechanism contributing to the
preservation of genomic stability by monitoring the microtubule attachment to, and/or the …

ProXL is a Web application and accompanying database designed for sharing, visualizing,
and analyzing bottom-up protein cross-linking mass spectrometry data with an emphasis on …

Accurate segregation of chromosomes to daughter cells is a critical aspect of cell division. It
requires the kinetochores on duplicated chromosomes to biorient, attaching to microtubules …