Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …

Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …

The easy and rapid spread of bacterial contamination and the risk it poses to human health
makes evident the need for analytical methods alternative to conventional time-consuming …

Plant parasitic nematodes (PPNs) cause an important class of diseases that occur in almost
all types of crops, seriously affecting yield and quality and causing great economic losses …

Multiplexed isothermal amplification and detection of nucleic acid sequences and
biomarkers is of increasing importance in diverse areas including advanced diagnostics …

The development of ultrasensitive, selective, simple, and rapid microRNA (miRNA) detection
strategies has been crucial because of their use as probable biomarkers for diagnosing …

Nucleic acid amplification tests (NAATs) are very sensitive and specific methods, but they
mainly rely on centralized laboratories and therefore are not suitable for point-of-care …

Recombinase polymerase amplification (RPA) is considered one of the best amplification
methods for realizing a miniaturized diagnostic instrument; however, it is notably challenging …

High-risk pathogens such as Francisella tularensis and Yersinia pestis are categorized as
highly hazardous organisms that can be used as biological weapons. Given the extreme …

The detection of genetic material (DNA or RNA) is becoming increasingly important not only
in the clinical practice but also in food quality control or environmental analysis. Since the …