Stimulated emission depletion microscopy

G Lukinavičius, J Alvelid, R Gerasimaitė… - Nature Reviews …, 2024 - nature.com
In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

Imaging brain tissue architecture across millimeter to nanometer scales

JM Michalska, J Lyudchik, P Velicky… - Nature …, 2024 - nature.com
Mapping the complex and dense arrangement of cells and their connectivity in brain tissue
demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels …

Fluorescence microscopy shadow imaging for neuroscience

VVGK Inavalli, V Puente Muñoz, JE Draffin… - Frontiers in Cellular …, 2024 - frontiersin.org
Fluorescence microscopy remains one of the single most widely applied experimental
approaches in neuroscience and beyond and is continuously evolving to make it easier and …

Light-microscopy based dense connectomic reconstruction of mammalian brain tissue

MR Tavakoli, J Lyudchik, M Januszewski, V Vistunou… - bioRxiv, 2024 - biorxiv.org
The information-processing capability of the brain's cellular network depends on the
physical wiring pattern between neurons and their molecular and functional characteristics …

Tidying up white matter: Neuroplastic transformations in sensorimotor tracts following slackline skill acquisition

K Koschutnig, B Weber, A Fink - Human Brain Mapping, 2024 - Wiley Online Library
This study investigated changes in white matter (WM) morphology following complex motor
learning, that is, the learning to walk a slackline. A sample of young adults from the general …

Shadow imaging for panoptical visualization of brain tissue in vivo

Y Dembitskaya, AKJ Boyce, A Idziak… - Nature …, 2023 - nature.com
Progress in neuroscience research hinges on technical advances in visualizing living brain
tissue with high fidelity and facility. Current neuroanatomical imaging approaches either …

3D super-resolution imaging of PSD95 reveals an abundance of diffuse protein supercomplexes in the mouse brain

S Daly, E Bulovaite, A Handa, K Morris… - ACS Chemical …, 2024 - ACS Publications
PSD95 is an abundant scaffolding protein that assembles multiprotein complexes controlling
synaptic physiology and behavior. Confocal microscopy has previously shown that PSD95 is …

Dense cell-by-cell systems of PDEs: approximation, spectral analysis, and preconditioning

P Benedusi, P Ferrari, M Causemann… - arXiv preprint arXiv …, 2024 - arxiv.org
In the present study, we consider the Extra-Membrane-Intra model (EMI) for the simulation of
excitable tissues at the cellular level. We provide the (possibly large) system of partial …

[HTML][HTML] Proximity adjusted centroid mapping for accurate detection of nuclei in dense 3D cell systems

T Van De Looverbosch, S De Beuckeleer… - Computers in Biology …, 2025 - Elsevier
In the past decade, deep learning algorithms have surpassed the performance of many
conventional image segmentation pipelines. Powerful models are now available for …

[HTML][HTML] Imaging the extracellular matrix in live tissues and organisms with a glycan-binding fluorophore

A Fiore, G Yu, JJ Northey, R Patel, TA Ravenscroft… - bioRxiv, 2024 - ncbi.nlm.nih.gov
All multicellular systems produce and dynamically regulate extracellular matrices (ECM) that
play important roles in both biochemical and mechanical signaling. Though the spatial …