Numerous biological processes are concurrently and coordinately active in every living cell.
Each of them encompasses synthetic, catalytic and regulatory functions that are, almost …

The question of how genomic information is expressed to determine phenotypes is of central
importance for basic and translational life science research and has been studied by …

Measuring precise concentrations of proteins can provide insights into biological processes.
Here we use efficient protein extraction and sample fractionation, as well as state-of-the-art …

The ability of living systems to adapt to changing conditions originates from their capacity to
change their molecular constitution. This is achieved by multiple mechanisms that modulate …

According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Pathogenic leptospires are the bacterial agents of leptospirosis, which is an emerging
zoonotic disease that affects animals and humans worldwide. The success of leptospires as …

Recent advances in next-generation DNA sequencing and proteomics provide an
unprecedented ability to survey mRNA and protein abundances. Such proteome-wide …

Absolute protein quantification using mass spectrometry (MS)-based proteomics delivers
protein concentrations or copy numbers per cell. Existing methodologies typically require a …

Gene expression is a multistep process that involves the transcription, translation and
turnover of messenger RNAs and proteins. Although it is one of the most fundamental …

Mass-spectrometry-based proteomics is continuing to make major contributions to the
discovery of fundamental biological processes and, more recently, has also developed into …