The lipid biochemistry of eukaryotic algae

Y Li-Beisson, JJ Thelen, E Fedosejevs… - Progress in Lipid …, 2019 - Elsevier
Algal lipid metabolism fascinates both scientists and entrepreneurs due to the large diversity
of fatty acyl structures that algae produce. Algae have therefore long been studied as …

Recent advancements in the genetic engineering of microalgae

AE Sproles, FJ Fields, TN Smalley, CH Le, A Badary… - Algal Research, 2021 - Elsevier
The development of more sustainable food, feed, and bio-products is critical to mitigating the
environmental stresses facing our world today. Algae, which includes seaweeds, eukaryotic …

A chloroplast protein atlas reveals punctate structures and spatial organization of biosynthetic pathways

L Wang, W Patena, KA Van Baalen, Y Xie, ER Singer… - Cell, 2023 - cell.com
Chloroplasts are eukaryotic photosynthetic organelles that drive the global carbon cycle.
Despite their importance, our understanding of their protein composition, function, and …

A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis

X Li, W Patena, F Fauser, RE Jinkerson, S Saroussi… - Nature …, 2019 - nature.com
Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their
protein-coding genes remain uncharacterized,. Characterization of these genes could be …

An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii

X Li, R Zhang, W Patena, SS Gang, SR Blum… - The Plant …, 2016 - academic.oup.com
The green alga Chlamydomonas reinhardtii is a leading unicellular model for dissecting
biological processes in photosynthetic eukaryotes. However, its usefulness has been limited …

Random mutagenesis as a promising tool for microalgal strain improvement towards industrial production

M Trovão, LM Schüler, A Machado, G Bombo… - Marine drugs, 2022 - mdpi.com
Microalgae have become a promising novel and sustainable feedstock for meeting the rising
demand for food and feed. However, microalgae-based products are currently hindered by …

Successful transient expression of Cas9 and single guide RNA genes in Chlamydomonas reinhardtii

W Jiang, AJ Brueggeman, KM Horken… - Eukaryotic …, 2014 - Am Soc Microbiol
The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has
become a powerful and precise tool for targeted gene modification (eg, gene knockout and …

Structure of the decorated ciliary doublet microtubule

M Ma, M Stoyanova, G Rademacher, SK Dutcher… - Cell, 2019 - cell.com
The axoneme of motile cilia is the largest macromolecular machine of eukaryotic cells. In
humans, impaired axoneme function causes a range of ciliopathies. Axoneme assembly …

[HTML][HTML] Structure of a TOC-TIC supercomplex spanning two chloroplast envelope membranes

Z Jin, L Wan, Y Zhang, X Li, Y Cao, H Liu, S Fan, D Cao… - Cell, 2022 - cell.com
The TOC and TIC complexes are essential translocons that facilitate the import of the
nuclear genome-encoded preproteins across the two envelope membranes of chloroplast …

Chlamydomonas as a model for biofuels and bio‐products production

MA Scranton, JT Ostrand, FJ Fields… - The Plant …, 2015 - Wiley Online Library
Developing renewable energy sources is critical to maintaining the economic growth of the
planet while protecting the environment. First generation biofuels focused on food crops like …