Two electrogenic H+‐pumps, the vacuolar type H+‐ATPase (V‐ATPase) and the vacuolar
pyrophosphatase, coexist at membranes of the secretory pathway of plants. The V‐ATPase …

The endoplasmic reticulum (ER) of plants is comprised of a three‐dimensional network of
continuous tubules and sheets that underlies the plasma membrane, courses through the …

The pH of intracellular compartments is essential for the viability of cells. Despite its
relevance, little is known about the pH of these compartments. To measure pH in vivo, we …

In eukaryotic cells, compartments of the highly dynamic endomembrane system are acidified
to varying degrees by the activity of vacuolar H+-ATPases (V-ATPases). In the Arabidopsis …

The circulation of ions across biological membranes is a fundamental process of cellular
energetics (Harold, 1986). Indeed, ion currents play the key role in energy capture during …

The plant cell vacuole has multiple functions, including storage of proteins and maintenance
of an acidic pH where proteases will have maximal activity. It has been assumed that these …

▪ Abstract Regulation of the contents and volume of vacuoles in plant cells depends on the
coordinated activities of transporters and channels located in the tonoplast (vacuolar …

In all multicellular organisms growth and morphogenesis must be coordinated, but for higher
plants, this is of particular importance because the timing of organogenesis is not fixed but …

Vacuoles are multifunctional organelles essential for the sessile lifestyle of plants. Despite
their central functions in cell growth, storage, and detoxification, knowledge about …

Plant cell vacuoles may have storage or lytic functions, but biochemical markers specific for
the tonoplasts of functionally distinct vacuoles are poorly defined. Here, we use antipeptide …