▪ Abstract Plant transformation is now a core research tool in plant biology and a practical
tool for cultivar improvement. There are verified methods for stable introduction of novel …

Agrobacterium tumefaciens has been routinely utilized in gene transfer to dicotyledonous
plants, but monocotyledonous plants including important cereals were thought to be …

Cultivated rice varieties are all diploid, and polyploidization of rice has long been desired
because of its advantages in genome buffering, vigorousness, and environmental …

Background Plant protoplasts, a proven physiological and versatile cell system, are widely
used in high-throughput analysis and functional characterization of genes. Green …

Here, we provide comprehensive, highly efficient protocols for Agrobacterium tumefaciens-
mediated transformation of a wide range of rice genotypes. Methods that use either …

A reproducible system for the generation of fertile, transgenic maize plants has been
developed. Cells from embryogenic maize suspension cultures were transformed with the …

We have obtained fertile transgenic wheat plants resistant to the broad spectrum herbicide
Basta®(active ingredient phosphinothricin, PPT) by high velocity microprojectile …

Abstract Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in
producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class …

Cytogenetics plays an important role in understanding the chromosomal and genetic
architecture of plant species. Plant Cytogenetics, Third Edition follows the tradition of its …

Nearly isogenic lines (NILs) of rice (Oryza sativa) differing at a locus conferring resistance to
the pathogen Xanthomonas oryzae pv. oryzae were surveyed with 123 DNA markers and …