Substrate specificity of aquatic extracellular peptidases assessed by competitive inhibition assays using synthetic substrates

AD Steen, JP Vazin, SM Hagen, KH Mulligan… - Aquatic Microbial …, 2015 - int-res.com
The identities and biochemical properties of extracellular enzymes present in natural
environments are poorly constrained. We used a series of competitive inhibition experiments …

Comparing extracellular enzymatic hydrolysis between plain peptides and their corresponding analogs in the northern Gulf of Mexico Mississippi River plume

S Liu, Z Liu - Marine Chemistry, 2015 - Elsevier
Peptide analogs, such as leucine-methylcoumarinylamide (Leu-MCA) and Lucifer yellow
anhydride-tetraalanine (LYA-Ala4), are often used as proxies to quantify extracellular …

Differentiating the role of different-sized microorganisms in peptide decomposition during incubations using size-fractioned coastal seawater

S Liu, A Riesen, Z Liu - Journal of Experimental Marine Biology and …, 2015 - Elsevier
Peptide decomposition by different-sized microorganisms was compared by incubating
tetrapeptide alanine–valine–phenylalanine–alanine (AVFA), a fragment of RuBisCO, in …

Extracellular proteases are released by ciliates in defined seawater microcosms

NV Thao, A Nozawa, Y Obayashi, SI Kitamura… - Marine Environmental …, 2015 - Elsevier
The biodegradation of proteins in seawater requires various proteases which are commonly
thought to be mainly derived from heterotrophic bacteria. We, however, found that protists …

Potential activities of freshwater exo-and endo-acting extracellular peptidases in East Tennessee and the Pocono Mountains

L Mullen… - Frontiers in …, 2018 - frontiersin.org
Proteins constitute a particularly bioavailable subset of organic carbon and nitrogen in
aquatic environments but must be hydrolyzed by extracellular enzymes prior to being …

[PDF][PDF] Potential Activities of Freshwater Exo-and Endo-Acting Extracellular Peptidases in East Tennessee and the Pocono Mountains

K Boerrigter, N Ferriero, J Rosalsky… - academia.edu
Proteins constitute a particularly bioavailable subset of organic carbon and nitrogen in
aquatic environments but must be hydrolyzed by extracellular enzymes prior to being …

Hydrolysis and decomposition of small peptides in marine environments

S Liu - 2016 - repositories.lib.utexas.edu
Proteins and peptides are important labile organic matter supporting growth of
microorganisms in seawater. Small peptides (< 600 Da) are key intermediates linking …