A simple, sensitive, specific and fast method based on the loop-mediated isothermal
amplification (LAMP) technique and cleavable molecular beacon (CMB) was developed for …

Real-time digital polymerase chain reaction (qdPCR) coupled with machine learning (ML)
methods has shown the potential to unlock scientific breakthroughs, particularly in the field …

Resource managers may be hesitant to make decisions based on environmental (e) DNA
results alone since eDNA is an indirect method of species detection. One way to reduce the …

The variable amplification efficiency of each thermal cycle of qPCR obeys the Poisson
distribution, and the qPCR system dynamically changes, so there must be a detection error …

Estimates of EC 50 from dose–response data play an important role in comparing drug
potencies. When the sampling data of dose–response studies fail to follow a sigmoidal …

The standard approach for quantitative estimation of genetic materials with qPCR is
calibration with known concentrations for the target substance, in which estimates of the …

Background: Laboratory diagnosis of Lyme borreliosis refers to some methods with known
limitations. Molecular diagnostics using specific nucleic acid probes may overcome some of …

We created a 2‐week, dual‐module summer course introducing high school students to
environmental toxicology by teaching them quantitative polymerase chain reaction (qPCR) …

Conclusion Manual classification of ACs is time-consuming and error-prone, especially with
large data sets where a significant proportion of curves deviate from sigmoidal shape, and …

Background We aimed to compare absolute plasma concentrations of N-terminal pro-brain
natriuretic peptide (NT-proBNP) and growth differentiation factor 15 (GDF-15) obtained by a …