The ligase chain reaction (LCR), as a classic nucleic acid amplification technique, is popular
in the detection of DNA and RNA due to its simplicity, powerfulness, and high specificity …

The development of non-invasive techniques for the diagnosis of cancer, characterization of
mutation and monitoring treatment response could greatly reduce the morbidity and mortality …

Although various methods have been developed to suffice the oligonucleotide demand of
molecular biology laboratories, in vitro production of high-purity ssDNAs remains to be a …

Accurate quantifications of cellular miRNAs are important not only for accelerating them
becoming reliable diagnostics biomarkers but also for deeply understanding their influence …

The tough challenges for the convenient and quantitative determination of circulating
miRNAs (cmiRNAs) in the peripheral blood are low abundance, high interference and lack …

In situ analysis of tumor-related messenger RNAs (mRNAs) is significant in identifying
cancer cells at the genetic level in the early stage. Rolling circle amplification (RCA)-based …

We report a symmetric exponential amplification-assisted fluorescence in situ hybridization
(SEXPAR-FISH) strategy for imaging intracellular-specific microRNAs (miRNAs) in cells. The …

A fluorescent biosensing strategy based on three helix molecular beacons (tMBs) for
simultaneous simple and rapid detection of HIV related genes was developed. This tMBs …

Currently, reported affinity pairings still lack in diversity, and thus terminal protection relying
on steric hindrance is restricted in designing nucleic acid-based analytical systems. In this …

A method for the ABO and Rhesus (Rh) blood group typing from individual erythrocytes is
proposed in this study. Blood-group-specific antibodies immobilized to gold nanoparticles …