Advances in ultra-high-resolution mass spectrometry for pharmaceutical analysis

E Deschamps, V Calabrese, I Schmitz, M Hubert-Roux… - Molecules, 2023 - mdpi.com
Pharmaceutical analysis refers to an area of analytical chemistry that deals with active
compounds either by themselves (drug substance) or when formulated with excipients (drug …

Dissecting the structural heterogeneity of proteins by native mass spectrometry

DJ Reid, S Thibert, M Zhou - Protein Science, 2023 - Wiley Online Library
A single gene yields many forms of proteins via combinations of posttranscriptional/
posttranslational modifications. Proteins also fold into higher‐order structures and interact …

Real-time biosynthetic reaction monitoring informs the mechanism of action of antibiotics

AO Oluwole, VM Hernández-Rocamora… - Journal of the …, 2024 - ACS Publications
The rapid spread of drug-resistant pathogens and the declining discovery of new antibiotics
have created a global health crisis and heightened interest in the search for novel …

Native Mass Spectrometry: Insights and Opportunities for Targeted Protein Degradation

LM Sternicki, SA Poulsen - Analytical Chemistry, 2023 - ACS Publications
Native mass spectrometry (nMS) is one of the most powerful biophysical methods for the
direct observation of noncovalent protein interactions with both small molecules and other …

[HTML][HTML] Native mass spectrometry-directed drug discovery: Recent advances in investigating protein function and modulation

F Fiorentino, D Rotili, A Mai - Drug Discovery Today, 2023 - Elsevier
Native mass spectrometry (nMS) is a biophysical method for studying protein complexes and
can provide insights into subunit stoichiometry and composition, protein–ligand, and protein …

Mass spectrometry of intact membrane proteins: shifting towards a more native-like context

A Oluwole, D Shutin, JR Bolla - Essays in Biochemistry, 2023 - portlandpress.com
Integral membrane proteins are involved in a plethora of biological processes including
cellular signalling, molecular transport, and catalysis. Many of these functions are mediated …

Enhanced declustering enables native top-down analysis of membrane protein complexes using ion-mobility time-aligned fragmentation

K Sokratous, DA Cooper-Shepherd… - Journal of the …, 2024 - ACS Publications
Native mass spectrometry (MS) is proving to be a disruptive technique for studying the
interactions of proteins, necessary for understanding the functional roles of these …

Decavanadate interactions with the elements of the SARS-CoV-2 spike protein highlight the potential role of electrostatics in disrupting the infectivity cycle

D Favre, JF Harmon, A Zhang, MS Miller… - Journal of Inorganic …, 2022 - Elsevier
Polyoxidometalates (POMs) exhibit a range of biological properties that can be exploited for
a variety of therapeutic applications. However, their potential utility as antivirals has been …

Differences in oligomerization of the SARS-CoV-2 envelope protein, poliovirus VP4, and HIV Vpu

JA Townsend, O Fapohunda, Z Wang, H Pham… - Biochemistry, 2024 - ACS Publications
Viroporins constitute a class of viral membrane proteins with diverse roles in the viral life
cycle. They can self-assemble and form pores within the bilayer that transport substrates …

Structural mass spectrometry approaches to understand multidrug efflux systems

B Russell Lewis, R Lawrence… - Essays in …, 2023 - portlandpress.com
Multidrug efflux pumps are ubiquitous across both eukaryotes and prokaryotes, and have
major implications in antimicrobial and multidrug resistance. They reside within cellular …