Molecular biologist's guide to proteomics

PR Graves, TAJ Haystead - Microbiology and molecular biology …, 2002 - Am Soc Microbiol
The emergence of proteomics, the large-scale analysis of proteins, has been inspired by the
realization that the final product of a gene is inherently more complex and closer to function …

Proteomics: the move to mixtures

J Peng, SP Gygi - Journal of mass spectrometry, 2001 - Wiley Online Library
Proteomics can be defined as the systematic analysis of proteins for their identity, quantity
and function. In contrast to a cell's static genome, the proteome is both complex and …

Unintended effects and their detection in genetically modified crops

F Cellini, A Chesson, I Colquhoun, A Constable… - Food and Chemical …, 2004 - Elsevier
The commercialisation of GM crops in Europe is practically non-existent at the present time.
The European Commission has instigated changes to the regulatory process to address the …

[HTML][HTML] Gene expression profiling: monitoring transcription and translation products using DNA microarrays and proteomics

JE Celis, M Kruhøffer, I Gromova, C Frederiksen… - FEBS letters, 2000 - Elsevier
Novel and powerful technologies such as DNA microarrays and proteomics have made
possible the analysis of the expression levels of multiple genes simultaneously both in …

[PDF][PDF] Perspectives for mass spectrometry and functional proteomics

J Godovac‐Zimmermann, LR Brown - Mass spectrometry reviews, 2001 - Citeseer
One of the most intellectually challenging, scientifically productive, and socially applicable
endeavors of modern biological science is the current effort to understand the function of …

Prefractionation techniques in proteome analysis: the mining tools of the third millennium

PG Righetti, A Castagna, P Antonioli… - Electrophoresis, 2005 - Wiley Online Library
The present review deals with prefractionation protocols used in proteomic investigation in
preparation for mass spectrometry (MS) or two‐dimensional electrophoresis (2‐DE) map …

Invited review: Toxicoproteomics: Proteomics applied to toxicology and pathology

BA Wetmore, BA Merrick - Toxicologic pathology, 2004 - journals.sagepub.com
Global measurement of proteins and their many attributes in tissues and biofluids defines
the field of proteomics. Toxicoproteomics, as part of the larger field of toxicogenomics, seeks …

Use of antibodies for detection of phosphorylated proteins separated by two‐dimensional gel electrophoresis

H Kaufmann, JE Bailey… - PROTEOMICS …, 2001 - Wiley Online Library
Protein phosphorylation and dephosphorylation are key regulatory mechanisms in
prokaryotic and eukaryotic cells. Considering the role of phosphorylation in many human …

2D or not 2D

SJ Fey, PM Larsen - Current Opinion in Chemical Biology, 2001 - Elsevier
2D gel electrophoresis is the technology that everyone loves to hate—it requires manual
dexterity and precision to reproduce precisely and is thus not well-suited as a high …

Cardiovascular proteomics: evolution and potential

DK Arrell, I Neverova, JE Van Eyk - Circulation research, 2001 - Am Heart Assoc
The development of proteomics is a timely one for cardiovascular research. Analyses at the
organ, subcellular, and molecular levels have revealed dynamic, complex, and subtle …