In RNA viruses, a small increase in their mutation rates can be sufficient to exceed their
threshold of viability. Lethal mutagenesis is a therapeutic strategy based on the use of …

In RNA viruses, a small increase in their mutation rates can be sufficient to exceed their
threshold of viability. Lethal mutagenesis is a therapeutic strategy based on the use of …

The high mutation rate of the human immunodeficiency virus type 1 (HIV-1) plays a major
role in treatment resistance, from the development of vaccines to therapeutic drugs. In …

Human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) has been the
primary interest among studies on antiviral discovery, viral replication kinetics, drug …

Studies of retroviruses have led to many extraordinary discoveries that have advanced our
understanding of not only human diseases, but also molecular biology as a whole. The most …

In M13mp2 lacZα forward mutation assays measuring intrinsic fidelity of DNA-dependent
DNA synthesis, wild-type human immunodeficiency virus type 1 (HIV-1) RTs of group …

Background There are few data on the prevalence of acquired drug resistance mutations
(ADRs) in Hunan Province, China, that could affect the effectiveness of antiretroviral therapy …

In this study, we devised a simple and rapid method to analyze fidelity of reverse
transcriptase (RT) using next-generation sequencing (NGS). The method comprises a cDNA …

Retroviruses, like other RNA viruses, mutate at very high rates and exist as genetically
heterogeneous populations. The error-prone activity of viral reverse transcriptase (RT) is …

Coupled with PCR, reverse transcriptases (RTs) have been widely used for RNA detection
and gene expression analysis. Increased thermostability and nucleic acid binding affinity are …