Summary Protein homeostasis, or" proteostasis," is indispensable for a balanced, healthy
environment within the cell. However, when natural proteostasis mechanisms are …

Molecular chaperones are highly conserved proteins that promote proper folding of other
proteins in vivo. Diverse chaperone systems assist de novo protein folding and trafficking …

Proteins perform many essential functions in biological systems and can be successfully
developed as bio-therapeutics. It is invaluable to be able to predict their properties based on …

Solid-state NMR spectroscopy (ssNMR) with magic-angle spinning (MAS) enables the
investigation of biological systems within their native context, such as lipid membranes, viral …

Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Recent advances in interrogating RNA folding dynamics have shown the classical model of
RNA folding to be incomplete. Here, we pose three prominent questions for the field that are …

Co-translational folding is a fundamental process for the efficient biosynthesis of nascent
polypeptides that emerge through the ribosome exit tunnel. To understand how this process …

Cells tightly regulate mRNA processing, localization, and stability to ensure accurate gene
expression in diverse cellular states and conditions. Most of these regulatory steps have …

Co-translational folding is crucial to ensure the production of biologically active proteins. The
ribosome can alter the folding pathways of nascent polypeptide chains, yet a structural …

The prevailing current view of protein folding is the thermodynamic hypothesis, under which
the native folded conformation of a protein corresponds to the global minimum of Gibbs free …