Comprehensive isolation of extracellular vesicles and nanoparticles

Q Zhang, DK Jeppesen, JN Higginbotham… - Nature protocols, 2023 - nature.com
There is an increasing appreciation for the heterogeneous nature of extracellular vesicles
(EVs). In addition, two nonvesicular extracellular nanoparticles (NVEPs), exomeres and …

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

H Tiernan, B Byrne, SG Kazarian - … Acta Part A: Molecular and Biomolecular …, 2020 - Elsevier
Abstract Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is
a label-free, non-destructive technique that can be applied to a vast range of biological …

Characterization of chitin and chitosan derived from Hermetia illucens, a further step in a circular economy process

M Triunfo, E Tafi, A Guarnieri, R Salvia, C Scieuzo… - Scientific Reports, 2022 - nature.com
Due to their properties and applications, the growing demand for chitin and chitosan has
stimulated the market to find more sustainable alternatives to the current commercial source …

Analyzing nanomaterial bioconjugates: a review of current and emerging purification and characterization techniques

KE Sapsford, KM Tyner, BJ Dair… - Analytical …, 2011 - ACS Publications
REVIEW be defined as the intersection of nanotechnology and biology and is loosely
characterized by two somewhat converse foci:(i) exploiting nanomaterials (NMs) to …

[HTML][HTML] Mechanistic enzymology in drug discovery: a fresh perspective

GA Holdgate, TD Meek, RL Grimley - Nature reviews Drug discovery, 2018 - nature.com
Given the therapeutic and commercial success of small-molecule enzyme inhibitors, as
exemplified by kinase inhibitors in oncology, a major focus of current drug-discovery and …

Quantitation of protein

JE Noble, MJA Bailey - Methods in enzymology, 2009 - Elsevier
The measurement of protein concentration in an aqueous sample is an important assay in
biochemistry research and development labs for applications ranging from enzymatic …

An analysis of critical factors for quantitative immunoblotting

KA Janes - Science signaling, 2015 - science.org
Immunoblotting (also known as Western blotting) combined with digital image analysis can
be a reliable method for analyzing the abundance of proteins and protein modifications, but …

Protein encapsulation using complex coacervates: what nature has to teach us

WC Blocher McTigue, SL Perry - Small, 2020 - Wiley Online Library
Protein encapsulation is a growing area of interest, particularly in the fields of food science
and medicine. The sequestration of protein cargoes is achieved using a variety of methods …

Analysis of umami taste substances of morel mushroom (Morchella sextelata) hydrolysates derived from different enzymatic systems

J Gao, D Fang, BM Kimatu, X Chen, X Wu, J Du… - Food Chemistry, 2021 - Elsevier
Seven enzyme groups were applied to hydrolyze broken fruiting bodies of morel mushroom
(Morchella sextelata) to extract umami substances. Physical-chemical properties, as well as …

Drug affinity responsive target stability (DARTS) accelerated small molecules target discovery: Principles and application

YS Ren, HL Li, XH Piao, ZY Yang, SM Wang… - Biochemical …, 2021 - Elsevier
Drug affinity responsive target stability (DARTS) is a novel target discovery approach and is
particularly adept at screening small molecule (SM) targets without requiring any structural …