Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical
technique that can be used to determine the structure of biological macromolecules and …

Colloidal nanoparticles doped with lanthanide ions can upconvert near-infrared light to
visible frequencies, enabling the application of such materials to biological imaging and …

Transmission electron microscopy (EM) is a versatile technique that can be used to image
biological specimens ranging from intact eukaryotic cells to individual proteins> 150 kDa …

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining
cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron …

The conserved co-activator complex Mediator enables regulated transcription initiation by
RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) …

Cryoelectron tomography provides unprecedented insights into the macromolecular and
supramolecular organization of cells in a close-to-living state. However because of the …

RNA polymerase (Pol) II produces messenger RNA during transcription of protein-coding
genes in all eukaryotic cells. The Pol II structure is known at high resolution from X-ray …

Transcription of eukaryotic protein-coding genes begins with assembly of the RNA
polymerase (Pol) II initiation complex and promoter DNA opening. Here we report cryo …

During transcription, RNA polymerase II (Pol II) associates with the conserved elongation
factor DSIF. DSIF renders the elongation complex stable and functions during Pol II pausing …

The 26S proteasome operates at the executive end of the ubiquitin-proteasome pathway.
Here, we present a cryo-EM structure of the Saccharomyces cerevisiae 26S proteasome at a …