Microtubules are long, slender polymers of αβ-tubulin found in all eukaryotic cells. Tubulins
associate longitudinally to form protofilaments, and adjacent protofilaments associate …

Eukaryogenesis, the origin of the eukaryotic cell, represents one of the fundamental
evolutionary transitions in the history of life on earth. This event, which is estimated to have …

Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …

Cryo-electron tomography (CET) is ideally suited for bridging the resolution gap between
molecular and cellular structural studies. However, CET is limited to a sample thickness …

Based on fluorescence microscopy, the actin homolog MreB has been thought to form
extended helices surrounding the cytoplasm of rod-shaped bacterial cells. The presence of …

Most, if not all, bacterial and archaeal cells contain at least one protein filament system.
Although these filament systems in some cases form structures that are very similar to …

Electron cryotomography (ECT) provides three-dimensional views of macromolecular
complexes inside cells in a native frozen–hydrated state. Over the last two decades, ECT …

Tubulins belong to the most abundant proteins in eukaryotes providing the backbone for
many cellular substructures like the mitotic and meiotic spindles, the intracellular …

Nanomaterials acquire a biomolecular corona upon introduction to biological media, leading
to biological transformations such as changes in protein function, unmasking of epitopes …

Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high.
Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to …