Live imaging is a powerful tool, enabling scientists to observe living organisms in real time.
In particular, when combined with fluorescence microscopy, live imaging allows the …

Technological advancements in biology and microscopy have empowered a transition from
bioimaging as an observational method to a quantitative one. However, as biologists are …

Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images.
Existing cell segmentation methods are often tailored to specific modalities or require …

Structural maintenance of chromosomes (SMC) protein complexes are essential for the
spatial organization of chromosomes. Whereas cohesin and condensin organize …

Spatiotemporal orchestration of gene expression is required for proper embryonic
development. The use of single-cell technologies has begun to provide improved resolution …

Across biological systems, cells undergo coordinated changes in gene expression, resulting
in transcriptome dynamics that unfold within a low-dimensional manifold. While low …

Understanding the complexity of cellular function within a tissue necessitates the
combination of multiple phenotypic readouts. Here, we developed a method that links …

Membrane tension is thought to be a long-range integrator of cell physiology. Membrane
tension has been proposed to enable cell polarity during migration through front-back …

Retrieving the complex responses of individual cells in the native three-dimensional tissue
context is crucial for a complete understanding of tissue functions. Here, we present …

A growing community is constructing a next-generation file format (NGFF) for bioimaging to
overcome problems of scalability and heterogeneity. Organized by the Open Microscopy …