All viable bacterial cells, whether they divide symmetrically or asymmetrically, must
coordinate their growth, division, cell volume and shape with the inheritance of the genome …

The DnaA protein has long been considered to play the key role in the initiation of
chromosome replication in modern bacteria. Many questions about this role, however …

In Escherichia coli, the replication initiator DnaA oscillates between an ATP-and an ADP-
bound state in a cell cycle-dependent manner, supporting regulation for chromosome …

In bacteria, DnaA is the most conserved DNA replication initiator protein. DnaA is a DNA
binding protein that is part of the AAA+ ATPase family. In addition to initiating chromosome …

The facultative aerobic bacterium Escherichia coli adjusts its cell cycle to environmental
conditions. Because of its lifestyle, the bacterium has to balance the use of oxygen with the …

The bacterium Escherichia coli contains a single circular chromosome with a defined
architecture. DNA replication initiates at a single origin called oriC. Two replication forks are …

Hydroxyurea (HU) is classified as a ribonucleotide reductase (RNR) inhibitor and has been
widely used to stall DNA replication by depleting deoxyribonucleoside triphosphate (dNTP) …

In all cells, initiation of chromosome replication depends on the activity of AAA+ initiator
proteins that form complexes with replication origin DNA. In bacteria, the conserved …

Expression of the Escherichia coli dnaN-encoded β clamp at≥ 10-fold higher than
chromosomally expressed levels impedes growth by interfering with DNA replication. We …

In Escherichia coli, like all organisms, DNA replication is coordinated with cell cycle
progression to ensure duplication of the genome prior to cell division. Chromosome …