[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

Opportunities and challenges in long-read sequencing data analysis

SL Amarasinghe, S Su, X Dong, L Zappia, ME Ritchie… - Genome biology, 2020 - Springer
Long-read technologies are overcoming early limitations in accuracy and throughput,
broadening their application domains in genomics. Dedicated analysis tools that take into …

[HTML][HTML] Nanopore sequencing and assembly of a human genome with ultra-long reads

M Jain, S Koren, KH Miga, J Quick, AC Rand… - Nature …, 2018 - nature.com
We report the sequencing and assembly of a reference genome for the human GM12878
Utah/Ceph cell line using the MinION (Oxford Nanopore Technologies) nanopore …

Nanopore sequencing: Review of potential applications in functional genomics

N Kono, K Arakawa - Development, growth & differentiation, 2019 - Wiley Online Library
Molecular biology has been led by various measurement technologies, and increased
throughput has developed omics analysis. The development of massively parallel …

High contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell

TP Michael, F Jupe, F Bemm, ST Motley… - Nature …, 2018 - nature.com
Abstract The handheld Oxford Nanopore MinION sequencer generates ultra-long reads with
minimal cost and time requirements, which makes sequencing genomes at the bench …

A world of opportunities with nanopore sequencing

RM Leggett, MD Clark - Journal of experimental botany, 2017 - academic.oup.com
Abstract Oxford Nanopore Technologies' MinION sequencer was launched in pre-release
form in 2014 and represents an exciting new sequencing paradigm. The device offers multi …

Deepbinner: Demultiplexing barcoded Oxford Nanopore reads with deep convolutional neural networks

RR Wick, LM Judd, KE Holt - PLoS computational biology, 2018 - journals.plos.org
Multiplexing, the simultaneous sequencing of multiple barcoded DNA samples on a single
flow cell, has made Oxford Nanopore sequencing cost-effective for small genomes …

MinIONQC: fast and simple quality control for MinION sequencing data

R Lanfear, M Schalamun, D Kainer, W Wang… - …, 2019 - academic.oup.com
MinIONQC provides rapid diagnostic plots and quality control data from one or more
flowcells of sequencing data from Oxford Nanopore Technologies' MinION instrument. It can …

Shotgun metagenome data of a defined mock community using Oxford Nanopore, PacBio and Illumina technologies

V Sevim, J Lee, R Egan, A Clum, H Hundley, J Lee… - Scientific data, 2019 - nature.com
Metagenomic sequence data from defined mock communities is crucial for the assessment
of sequencing platform performance and downstream analyses, including assembly, binning …

Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing

SST Mak, S Gopalakrishnan, C Carøe, C Geng… - …, 2017 - academic.oup.com
Ancient DNA research has been revolutionized following development of next-generation
sequencing platforms. Although a number of such platforms have been applied to ancient …