Genome-wide unbiased identification of double-stranded breaks enabled by sequencing
(GUIDE-seq) is a sensitive, unbiased, genome-wide method for defining the activity of …

Traditional therapeutic approaches such as chemotherapy and radiation therapy have
burdened cancer patients with onerous physical and psychological challenges …

Cys2His2 zinc finger (ZF) domains engineered to bind specific target sequences in the
genome provide an effective strategy for programmable regulation of gene expression, with …

Base editing in nuclear DNA and mitochondrial DNA (mtDNA) is broadly useful for
biomedical research, medicine, and biotechnology. Here, we present a base editing …

Zinc-finger nuclease (ZFN)-based in vivo genome editing is a novel treatment that can
potentially provide lifelong protein replacement with single intravenous administration …

The last two decades have marked significant advancement in the genome editing field.
Three generations of programmable nucleases (ZFNs, TALENs, and CRISPR-Cas system) …

Current treatments for chronic pain rely largely on opioids despite their substantial side
effects and risk of addiction. Genetic studies have identified in humans key targets pivotal to …

Environmental stresses such as salinity, drought, heat, freezing, heavy metal and even
pathogen infections seriously threaten the growth and yield of important cereal crops …

Recent advances in programmable nucleases including meganucleases (MNs), zinc finger
nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered …

Chromatin adopts different configurations that are regulated by reversible covalent
modifications, referred to as epigenetic marks. Epigenetic inhibitors have been approved for …