There has been a significant increase in the use of hydrophilic interaction liquid
chromatography (HILIC) to separate oligonucleotides. This rise in the use of HILIC has …

The polar nature of nucleobases, nucleosides and nucleotides makes hydrophilic interaction
chromatography (HILIC) a good choice of technology for separation. Both naturally occurring …

Large synthetic oligonucleotides such as guide ribonucleic acid (gRNA), a critical reagent in
clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 genome editing …

A surge in the approval of therapeutic oligonucleotides since 2016 has made this class of
chemical modalities an essential toolbox for addressing unmet medical needs in the realm …

Short interfering RNA (siRNA) represents a rapidly expanding class of marketed
oligonucleotide therapeutics. Due to its double-stranded nature, the characterization of …

Therapeutic oligonucleotides are a novel chemical modality with unique characteristics that
offer significant therapeutic potential. With the latest surge in development, they are paving …

The pharmaceutical industry is rapidly advancing toward new drug modalities, necessitating
the development of advanced analytical strategies for effective, meaningful, and reliable …

Therapeutic oligonucleotides (ONs) are a new generation of drugs that have captured the
attention of the pharmaceutical industry over the past decade, as evidenced by the FDA …

Single-stranded guide RNAs (sgRNAs) are important therapeutic modalities that facilitate
selective genome editing by the CRISPR/Cas9 system. While these therapeutic modalities …

Phosphorothioate (PS) modifications in single-guided RNA (sgRNA) are crucial for genome
editing applications using the CRISPR/Cas9 system. These modifications may enhance …