Small molecule inhibitors that target components of the spliceosome have great potential as
tools to probe splicing mechanism and dissect splicing regulatory networks in cells. These …

In eukaryotic cells, introns are spliced from pre-mRNAs by the spliceosome. Both the
composition and the structure of the spliceosome are highly dynamic, and eight DExD/H …

Spliceosome rearrangements facilitated by RNA helicase PRP16 before catalytic step two of
splicing are poorly understood. Here we report a 3D cryo-electron microscopy structure of …

Little is known about the spliceosome's structure before its extensive remodeling into a
catalytically active complex. Here, we report a 3D cryo-EM structure of a pre-catalytic human …

Abstract U4/U6. U5 tri-snRNP is a 1.5-megadalton pre-assembled spliceosomal complex
comprising U5 small nuclear RNA (snRNA), extensively base-paired U4/U6 snRNAs and …

The prespliceosome, comprising U1 and U2 small nuclear ribonucleoproteins (snRNPs)
bound to the precursor messenger RNA 5ʹ splice site (5ʹSS) and branch point sequence …

The U4/U6. U5 triple small nuclear ribonucleoprotein (tri-snRNP) is a major spliceosome
building block. We obtained a three-dimensional structure of the 1.8-megadalton human tri …

INTRODUCTION The spliceosome, including its catalytic center, is formed anew on each pre-
mRNA intron, through a pathway involving multiple, successive assembly intermediates …

The U2 small nuclear ribonucleoprotein (snRNP) has an essential role in the selection of the
precursor mRNA branch-site adenosine, the nucleophile for the first step of splicing. Stable …

During exon ligation, the Saccharomyces cerevisiae spliceosome recognizes the 3′-splice
site (3′ SS) of precursor messenger RNA (pre-mRNA) through non–Watson-Crick pairing …