Ribosome-targeting antibiotics and mechanisms of bacterial resistance

DN Wilson - Nature Reviews Microbiology, 2014 - nature.com
The ribosome is one of the main antibiotic targets in the bacterial cell. Crystal structures of
naturally produced antibiotics and their semi-synthetic derivatives bound to ribosomal …

A practical guide to single-molecule FRET

R Roy, S Hohng, T Ha - Nature methods, 2008 - nature.com
Single-molecule fluorescence resonance energy transfer (smFRET) is one of the most
general and adaptable single-molecule techniques. Despite the explosive growth in the …

Structural basis of early translocation events on the ribosome

EJ Rundlet, M Holm, M Schacherl, SK Natchiar… - Nature, 2021 - nature.com
Peptide-chain elongation during protein synthesis entails sequential aminoacyl-tRNA
selection and translocation reactions that proceed rapidly (2–20 per second) and with a low …

What recent ribosome structures have revealed about the mechanism of translation

TM Schmeing, V Ramakrishnan - Nature, 2009 - nature.com
The high-resolution structures of ribosomal subunits published in 2000 have revolutionized
the field of protein translation. They facilitated the determination and interpretation of …

An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments

CE Aitken, RA Marshall, JD Puglisi - Biophysical journal, 2008 - cell.com
The application of single-molecule fluorescence techniques to complex biological systems
places demands on the performance of single fluorophores. We present an enzymatic …

Aminoglycoside interactions and impacts on the eukaryotic ribosome

I Prokhorova, RB Altman… - Proceedings of the …, 2017 - National Acad Sciences
Aminoglycosides are chemically diverse, broad-spectrum antibiotics that target functional
centers within the bacterial ribosome to impact all four principle stages (initiation, elongation …

Distinct stages of the translation elongation cycle revealed by sequencing ribosome-protected mRNA fragments

LF Lareau, DH Hite, GJ Hogan, PO Brown - elife, 2014 - elifesciences.org
During translation elongation, the ribosome ratchets along its mRNA template, incorporating
each new amino acid and translocating from one codon to the next. The elongation cycle …

Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP

CE Carbone, AB Loveland, HB Gamper Jr… - Nature …, 2021 - nature.com
During translation, a conserved GTPase elongation factor—EF-G in bacteria or eEF2 in
eukaryotes—translocates tRNA and mRNA through the ribosome. EF-G has been proposed …

mRNA reading frame maintenance during eukaryotic ribosome translocation

N Milicevic, L Jenner, A Myasnikov, M Yusupov… - Nature, 2024 - nature.com
One of the most critical steps of protein synthesis is coupled translocation of messenger
RNA (mRNA) and transfer RNAs (tRNAs) required to advance the mRNA reading frame by …

Learning rates and states from biophysical time series: a Bayesian approach to model selection and single-molecule FRET data

JE Bronson, J Fei, JM Hofman, RL Gonzalez… - Biophysical journal, 2009 - cell.com
Time series data provided by single-molecule Förster resonance energy transfer (smFRET)
experiments offer the opportunity to infer not only model parameters describing molecular …