Protein analysis by shotgun/bottom-up proteomics
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …
Clinical potential of mass spectrometry-based proteogenomics
Cancer genomics research aims to advance personalized oncology by finding and targeting
specific genetic alterations associated with cancers. In genome-driven oncology, treatments …
specific genetic alterations associated with cancers. In genome-driven oncology, treatments …
Global detection of human variants and isoforms by deep proteome sequencing
An average shotgun proteomics experiment detects approximately 10,000 human proteins
from a single sample. However, individual proteins are typically identified by peptide …
from a single sample. However, individual proteins are typically identified by peptide …
Six alternative proteases for mass spectrometry–based proteomics beyond trypsin
Protein digestion using a dedicated protease represents a key element in a typical mass
spectrometry (MS)-based shotgun proteomics experiment. Up to now, digestion has been …
spectrometry (MS)-based shotgun proteomics experiment. Up to now, digestion has been …
Interpretation of shotgun proteomic data
AI Nesvizhskii, R Aebersold - Molecular & cellular proteomics, 2005 - ASBMB
The shotgun proteomic strategy based on digesting proteins into peptides and sequencing
them using tandem mass spectrometry and automated database searching has become the …
them using tandem mass spectrometry and automated database searching has become the …
Value of using multiple proteases for large-scale mass spectrometry-based proteomics
Large-scale protein sequencing methods rely on enzymatic digestion of complex protein
mixtures to generate a collection of peptides for mass spectrometric analysis. Here we …
mixtures to generate a collection of peptides for mass spectrometric analysis. Here we …
Protein digestion: an overview of the available techniques and recent developments
L Switzar, M Giera, WMA Niessen - Journal of proteome research, 2013 - ACS Publications
Several proteomics approaches are available that are defined by the level (protein or
peptide) at which analysis takes place. The most widely applied method still is bottom-up …
peptide) at which analysis takes place. The most widely applied method still is bottom-up …
Large-scale quantitative assessment of different in-solution protein digestion protocols reveals superior cleavage efficiency of tandem Lys-C/trypsin proteolysis over …
The complete and specific proteolytic cleavage of protein samples into peptides is crucial for
the success of every shotgun LC–MS/MS experiment. In particular, popular peptide-based …
the success of every shotgun LC–MS/MS experiment. In particular, popular peptide-based …
Enzyme immobilization on electrospun polymer nanofibers: An overview
Enzyme immobilization has attracted continuous attention in the fields of fine chemistry,
biomedicine, and biosensor. The performance of immobilized enzyme largely depends on …
biomedicine, and biosensor. The performance of immobilized enzyme largely depends on …
Consecutive proteolytic digestion in an enzyme reactor increases depth of proteomic and phosphoproteomic analysis
JR Wisniewski, M Mann - Analytical chemistry, 2012 - ACS Publications
Analytical advantages of using multiple enzymes for sample digestion (MED), primarily an
increase of sequence coverage, have been reported in several studies. However, this …
increase of sequence coverage, have been reported in several studies. However, this …