Glycoconjugates: synthesis, functional studies, and therapeutic developments

SS Shivatare, VS Shivatare, CH Wong - Chemical reviews, 2022 - ACS Publications
Glycoconjugates are major constituents of mammalian cells that are formed via covalent
conjugation of carbohydrates to other biomolecules like proteins and lipids and often …

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

M Ahmad, M Hirz, H Pichler, H Schwab - Applied microbiology and …, 2014 - Springer
Pichia pastoris is an established protein expression host mainly applied for the production of
biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished …

[HTML][HTML] Cultivation strategies to enhance productivity of Pichia pastoris: a review

V Looser, B Bruhlmann, F Bumbak, C Stenger… - Biotechnology …, 2015 - Elsevier
Pichia pastoris, a methylotrophic yeast, is an established system for the production of
heterologous proteins, particularly biopharmaceuticals and industrial enzymes. To maximise …

[HTML][HTML] Metabolic engineering of Pichia pastoris

DA Peña, B Gasser, J Zanghellini, MG Steiger… - Metabolic …, 2018 - Elsevier
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Improving industrial yeast strains: exploiting natural and artificial diversity

J Steensels, T Snoek, E Meersman… - FEMS microbiology …, 2014 - academic.oup.com
Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …

Harnessing yeast organelles for metabolic engineering

SK Hammer, JL Avalos - Nature chemical biology, 2017 - nature.com
Each subcellular compartment in yeast offers a unique physiochemical environment and
metabolite, enzyme, and cofactor composition. While yeast metabolic engineering has …

Production of recombinant proteins by yeast cells

E Çelik, P Çalık - Biotechnology advances, 2012 - Elsevier
Yeasts are widely used in production of recombinant proteins of medical or industrial
interest. For each individual product, the most suitable expression system has to be …

[HTML][HTML] Genome sequence of the recombinant protein production host Pichia pastoris

K De Schutter, YC Lin, P Tiels, A Van Hecke… - Nature …, 2009 - nature.com
The methylotrophic yeast Pichia pastoris is widely used for the production of proteins and as
a model organism for studying peroxisomal biogenesis and methanol assimilation. P …

Advances in the production of human therapeutic proteins in yeasts and filamentous fungi

TU Gerngross - Nature biotechnology, 2004 - nature.com
Yeast and fungal protein expression systems are used for the production of many industrially
relevant enzymes, and are widely used by the research community to produce proteins that …

Protein secretion in Pichia pastoris and advances in protein production

LM Damasceno, CJ Huang, CA Batt - Applied microbiology and …, 2012 - Springer
Yeast expression systems have been successfully used for over 20 years for the production
of recombinant proteins. With the growing interest in recombinant protein expression for …