Monoclonal antibodies (mAbs) are often selected from antigen-specific single B cells
derived from different hosts, which are notably short-lived in ex vivo culture conditions and …

Antibodies against foreign antigens are a critical component of the overall immune response
and can facilitate pathogen clearance during a primary infection and also protect against …

We present a technology to screen millions of B cells for natively paired human antibody
repertoires. Libraries of natively paired, variable region heavy and light (VH: VL) amplicons …

In recent years, major efforts have been made to develop sophisticated experimental and
bioinformatic workflows for sequencing adaptive immune repertoires. The immunological …

Monoclonal antibody discovery and engineering is a field that has traditionally been
dominated by high‐throughput screening platforms (eg hybridomas and surface display). In …

High-throughput antibody repertoire sequencing (Ig-seq) provides quantitative molecular
information on humoral immunity. However, Ig-seq is compromised by biases and errors …

Eight viruses are currently assigned to the family Filoviridae. Marburg virus, Sudan virus
and, in particular, Ebola virus have received the most attention both by researchers and the …

Highlights•Deep sequencing permits examination of local protein fitness landscapes.•NGS
aids in engineering affinity and specificity for protein molecular recognition.•Application to …

Antibodies are vital proteins of the immune system that recognize potentially harmful
molecules and initiate their removal. Mammals can efficiently create vast numbers of …

Background Recent technological advances in immune repertoire sequencing have created
tremendous potential for advancing our understanding of adaptive immune response …