Super-resolution microscopy (SRM) bypasses the diffraction limit, a physical barrier that
restricts the optical resolution to roughly 250 nm and was previously thought to be …

Fluorescence nanoscopy uniquely combines minimally invasive optical access to the
internal nanoscale structure and dynamics of cells and tissues with molecular detection …

Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

Stimulated emission depletion (STED) nanoscopy is a promising fluorescence microscopy to
detect unresolvable structures at the nanoscale level and then achieve a superior imaging …

Super-resolution microscopy depends on steps that can contribute to the formation of image
artifacts, leading to misinterpretation of biological information. We present NanoJ …

Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …

Live-cell super-resolution microscopy enables the imaging of biological structure dynamics
below the diffraction limit. Here we present enhanced super-resolution radial fluctuations …

Compartmentalized biochemical activities are essential to all cellular processes, but there is
no generalizable method to visualize dynamic protein activities in living cells at a resolution …

Studying the ultra-fine structure and functions of mitochondria at a nanoscale level has
garnered tremendous attention from biologists. Mitochondria perform many more functions …

Microscopic evaluation of resected tissue plays a central role in the surgical management of
cancer. Because optical microscopes have a limited depth-of-field (DOF), resected tissue is …