Several proteomics approaches are available that are defined by the level (protein or
peptide) at which analysis takes place. The most widely applied method still is bottom-up …

Meat is a valuable protein source with a balanced composition of essential amino acids and
various nutrients. This review aims to identify methods to improve digestion of meat proteins …

Enzymatic digestion for protein sequencing usually requires much time, and does not
always result in high sequence coverage. Here we report the use of aqueous microdroplets …

Improved in-solution tryptic digestion of proteins in terms of speed and peptide coverage
was achieved with the aid of a novel acid-labile anionic surfactant (ALS). Unlike SDS, ALS …

Milk fat globule membranes (MFGM) were isolated from the milk of mid-lactation Holstein
cows. The purified MFGM were fractionated using 1-dimensional SDS gels. Tryptic peptides …

The rate of protein digestion imposes significant limitations on high-throughput protein
identification using mass spectrometry. In this report, we demonstrate that proteins are …

Mass spectrometry-based identification of the components of multiprotein complexes often
involves solution-phase proteolytic digestion of the complex. The affinity purification of …

Peptide mapping analysis is a regulatory expectation to verify the primary structure of a
recombinant product sequence and to monitor post-translational modifications (PTMs) …

Shotgun proteomics, using amine-reactive isobaric tags (iTRAQ), was used to quantify
protein changes in milk fat globule membranes (MFGM) that were isolated from d 1 …

In this chapter we outline the state-of-the-art of extraction and fractionation techniques for
proteomic analysis of food proteins and peptides. The relevance of food sample preparation …