Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …

At the nuclear periphery, associations of chromatin with the nuclear lamina through lamina-
associated domains (LADs) aid functional organization of the genome. We review the …

Nuclei require a precise three-and four-dimensional organization of DNA to establish cell-
specific gene-expression programs. Underscoring the importance of DNA topology …

The binding of the transcriptional regulator CTCF to the genome has been implicated in the
formation of topologically associated domains (TADs). However, the general mechanisms of …

Fluorescence in situ hybridization (FISH) is the gold standard for visualizing genomic DNA in
fixed cells and tissues, but it is incompatible with live-cell imaging, and its combination with …

Mammalian genomes encode tens of thousands of long noncoding RNAs (lncRNAs) that
have been implicated in a diverse array of biological processes and human diseases. In …

The lack of efficient tools to image non-repetitive genes in living cells has limited our ability
to explore the functional impact of the spatiotemporal dynamics of such genes. Here, we …

The spatio-temporal organization of chromatin in the eukaryotic cell nucleus is of vital
importance for transcription, DNA replication and genome maintenance. Each of these …

CRISPR technology is a universal tool for genome engineering that has revolutionized
biotechnology. Recently identified unique CRISPR/Cas systems, as well as re-engineered …