In order to study the differential protein expression in complex biological samples, strategies
for rapid, highly reproducible and accurate quantification are necessary. Isotope labeling …

Spectral count, defined as the total number of spectra identified for a protein, has gained
acceptance as a practical, label-free, semiquantitative measure of protein abundance in …

Background Microenvironment cues involved in melanoma progression are largely
unknown. Melanoma is highly influenced in its aggressive phenotype by the changes it …

Background Following amputation, urodele salamander limbs reprogram somatic cells to
form a blastema that self-organizes into the missing limb parts to restore the structure and …

Formalin-fixed paraffin-embedded (FFPE) tissues are a real treasure for retrospective
analysis considering the amount of samples present in hospital archives, combined with …

Annotated formalin-fixed, paraffin-embedded (FFPE) tissue archives constitute a valuable
resource for retrospective biomarker discovery. However, proteomic exploration of archival …

Metastatic renal cell carcinoma (RCC) is one of the most treatment-resistant malignancies,
and patients have a dismal prognosis, with a< 10% five-year survival rate. The identification …

Metastatic melanoma, the primary cause of skin cancer-related death, warrants new
diagnostic and therapeutic approaches that target the regulatory machinery at molecular …

The clinical success of trastuzumab in breast cancer taught us that appropriate tumor
evaluation is mandatory for the correct identification of patients eligible for targeted …

Background Analysis of key therapeutic targets such as epidermal growth factor receptor
(EGFR) in clinical tissue samples is typically done by immunohistochemistry (IHC) and is …