Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is an essential electron
donor in all organisms. It provides the reducing power that drives numerous anabolic …

To understand complex biological systems requires the integration of experimental and
computational research—in other words a systems biology approach. Computational …

Abstract eQuilibrator (equilibrator. weizmann. ac. il) is a database of biochemical equilibrium
constants and Gibbs free energies, originally designed as a web-based interface. While the …

For over 10 years, ModelSEED has been a primary resource for the construction of draft
genome-scale metabolic models based on annotated microbial or plant genomes. Now …

Contrary to the textbook portrayal of glycolysis as a single pathway conserved across all
domains of life, not all sugar-consuming organisms use the canonical Embden–Meyerhoff …

Highlights•Multiple levels of metabolic engineering can be defined, from 'copy and paste'to
various forms of 'synthetic metabolism'.•The biochemical solution space for 'synthetic …

In metabolism research, thermodynamics is usually used to determine the directionality of a
reaction or the feasibility of a pathway. However, the relationship between thermodynamic …

Many carbon-fixing bacteria rely on a CO2 concentrating mechanism (CCM) to elevate the
CO2 concentration around the carboxylating enzyme ribulose bisphosphate carboxylase …

Standard Gibbs energies of reactions are increasingly being used in metabolic modeling for
applying thermodynamic constraints on reaction rates, metabolite concentrations and kinetic …

We present a quantitative model for sulfur isotope fractionation accompanying bacterial and
archaeal dissimilatory sulfate respiration. By incorporating independently available …