Design and analysis of CRISPR–Cas experiments
A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
manipulation of genomes in both targeted and large-scale experiments. Numerous software …
Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools
J Dronina, U Samukaite-Bubniene… - Journal of …, 2022 - Springer
Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …
Mechanistic Insights into the cis-and trans-Acting DNase Activities of Cas12a
Summary CRISPR-Cas12a (Cpf1) is an RNA-guided DNA-cutting nuclease that has been
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
repurposed for genome editing. Upon target DNA binding, Cas12a cleaves both the target …
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
Y Zhang, Q Ren, X Tang, S Liu, AA Malzahn… - Nature …, 2021 - nature.com
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
Kinetic basis for DNA target specificity of CRISPR-Cas12a
Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …
applications in human health and disease. However, DNA cleavage at off-target sites that …
Optimization of AsCas12a for combinatorial genetic screens in human cells
Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic
perturbations because they can process multiple guide RNAs expressed as a single …
perturbations because they can process multiple guide RNAs expressed as a single …
Cas9 versus Cas12a/Cpf1: Structure–function comparisons and implications for genome editing
Cas9 and Cas12a are multidomain CRISPR‐associated nucleases that can be programmed
with a guide RNA to bind and cleave complementary DNA targets. The guide RNA …
with a guide RNA to bind and cleave complementary DNA targets. The guide RNA …
Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
AA Malzahn, X Tang, K Lee, Q Ren, S Sretenovic… - BMC biology, 2019 - Springer
Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …
Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA
A Ferenczi, DE Pyott, A Xipnitou… - Proceedings of the …, 2017 - National Acad Sciences
The green alga Chlamydomonas reinhardtii is an invaluable reference organism to research
fields including algal, plant, and ciliary biology. Accordingly, decades-long standing …
fields including algal, plant, and ciliary biology. Accordingly, decades-long standing …
Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nuclease
Y Wang, Y Wang, D Pan, H Yu, Y Zhang, W Chen, F Li… - Cell Reports, 2022 - cell.com
Gene therapy is limited by inefficient delivery of large clustered regularly interspaced short
palindromic repeat (CRISPR) effectors, such as Cas9 and Cas12a nucleases. Cas12f …
palindromic repeat (CRISPR) effectors, such as Cas9 and Cas12a nucleases. Cas12f …