Real-time PCR quantification of spliced X-box binding protein 1 (XBP1) using a universal primer method

SB Yoon, YH Park, SA Choi, HJ Yang, PS Jeong… - PloS one, 2019 - journals.plos.org
X-box binding protein 1 (XBP1) mRNA processing plays a crucial role in the unfolded
protein response (UPR), which is activated in response to endoplasmic reticulum (ER) …

Effects of vitrification on the blastocyst gene expression profile in a porcine model

C Cuello, CA Martinez, JM Cambra, I Parrilla… - International Journal of …, 2021 - mdpi.com
This study was designed to investigate the impact of vitrification on the transcriptome profile
of blastocysts using a porcine (Sus scrofa) model and a microarray approach. Blastocysts …

From stability to reliability: Unveiling the un-biased reference genes in porcine ovarian granulosa cells under different conditions

Y Huo, X Li, C Sun, Z Pan, Q Li, X Du - Gene, 2024 - Elsevier
Selection of optimal reference genes (RGs) is fundamental for functional genomics studies
and gene expression analysis, which are two main approaches to identify functional genes …

Insensitive Effects of Inflammatory Cytokines on the Reference Genes of Synovial Fluid Resident-Mesenchymal Stem Cells Derived from Rheumatoid Arthritis Patients

EY Bok, SB Kim, G Thakur, YH Choe, SJ Oh… - International Journal of …, 2023 - mdpi.com
Mesenchymal stem cells derived from rheumatoid arthritis patients (RA-MSCs) provide an
understanding of a variety of cellular and immunological responses within the inflammatory …

Simultaneous inhibition of histone deacetylases and RNA synthesis enables totipotency reprogramming in pig SCNT embryos

MP de Macedo, WG Glanzner, K Gutierrez… - International Journal of …, 2022 - mdpi.com
Combining somatic cell nuclear transfer (SCNT) with genome editing technologies has
emerged as a powerful platform for the creation of unique swine lineages for agricultural and …

PPIA, HPRT1, and YWHAZ genes are suitable for normalization of mRNA expression in long‐term expanded human mesenchymal stem cells

RH Jeon, WJ Lee, YB Son, D Bharti… - BioMed Research …, 2019 - Wiley Online Library
Long‐term expansion of mesenchymal stem cells (MSCs) under defined culture conditions
is necessary in human stem cell therapy. However, it alters the characteristics of MSCs …

Cocultured porcine granulosa cells respond to excess non-esterified fatty acids during in vitro maturation

M Shi, MA Sirard - Journal of Ovarian Research, 2021 - Springer
Background Non-esterified fatty acids (NEFAs) are one of the main lipid components of
follicular fluid at concentrations that depend on circulating levels. Elevated levels of NEFAs …

Vitrification Effects on the Transcriptome of in vivo-Derived Porcine Morulae

C Cuello, CA Martinez, JM Cambra… - Frontiers in Veterinary …, 2021 - frontiersin.org
Despite the reported promising farrowing rates after non-surgical and surgical transfers of
vitrified porcine morulae and blastocysts produced in vivo (range: 70–75%), the pregnancy …

[HTML][HTML] PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic–pituitary–gonadal axis in sows

HD Kim, CH Jo, YH Choe, HJ Lee, M Jang… - Animal …, 2022 - ncbi.nlm.nih.gov
Objective The quantitative reverse transcription polymerase chain reaction (qPCR) is the
most accurate and reliable technique for analysis of gene expression. Endogenous …

Effects of NEFAs during IVM on pig embryos from granulosa cell‐cocultured oocytes

M Shi, MA Sirard - Molecular Reproduction and Development, 2021 - Wiley Online Library
Circulating levels of nonesterified fatty acids (NEFAs) are elevated in some females, which
can impair oocyte maturation and embryo development, and may alter the phenotype of the …