Construction of a Super-Folder Fluorescent Protein-Guided Secretory Expression System for the Production of Phospholipase D in Bacillus subtilis

H Zhang, X Li, Q Liu, J Sun, F Secundo… - Journal of agricultural …, 2021 - ACS Publications
Phospholipids (PLs) are one of the main ingredients in food and nutraceutical, cosmetics,
agriculture, and pharmaceutical products. Phospholipase D (PLD) is a crucial enzyme for …

[HTML][HTML] A contradictory action of procoagulant ficin by a fibrinolytic serine protease from Egyptian Ficus carica latex

MB Hamed, MO El-Badry, EI Kandil, IH Borai… - Biotechnology …, 2020 - Elsevier
Ficus carica is one of the most popular and edible plants. Its trees emanate latex of high
medical importance. The well-studied procoagulant effect of ficin is a hallmark of this latex …

Localization of Pyranose 2-Oxidase from Kitasatospora aureofaciens: A Step Closer to Elucidate a Biological Role

LJ Virginia, C Peterbauer - International Journal of Molecular Sciences, 2023 - mdpi.com
Lignin degradation in fungal systems is well characterized. Recently, a potential for lignin
depolymerization and modification employing similar enzymatic activities by bacteria is …

Characterization of Amycolatopsis 75iv2 dye-decolorizing peroxidase on O-glycosides

S Välimets, P Sun, LJ Virginia… - Applied and …, 2024 - Am Soc Microbiol
Dye-decolorizing peroxidases are heme peroxidases with a broad range of substrate
specificity. Their physiological function is still largely unknown, but a role in the …

Development of a thiostrepton-free system for stable production of PLD in Streptomyces lividans SBT5

J Wang, H Zhu, H Shang, B Guo, M Zhang… - Microbial Cell …, 2022 - Springer
Abstract Background Phospholipase D (PLD) is highly valuable in the food and medicine
industries, where it is used to convert low-cost phosphatidylcholine into high-value …

[HTML][HTML] UNC-10/SYD-2 links kinesin-3 to RAB-3-containing vesicles in the absence of the motor's PH domain

O Bayansan, P Bhan, CY Chang, SN Barmaver… - Neurobiology of …, 2025 - Elsevier
Abstract Kinesin-3 KIF1A (UNC-104 in C. elegans) is the major axonal transporter of
synaptic vesicles and mutations in this molecular motor are linked to KIF1A-associated …

Enhanced protein secretion in reduced genome strains of Streptomyces lividans

MB Hamed, T Busche, K Simoens, S Carpentier… - Microbial Cell …, 2024 - Springer
Background S. lividans TK24 is a popular host for the production of small molecules and the
secretion of heterologous protein. Within its large genome, twenty-nine non-essential …

Generation of a soluble form of human endoglin fused to green fluorescent protein

L Ruiz-Llorente, MC Vega, FJ Fernández… - International Journal of …, 2021 - mdpi.com
Endoglin (Eng, CD105) is a type I membrane glycoprotein that functions in endothelial cells
as an auxiliary receptor for transforming growth factor β (TGF-β)/bone morphogenetic protein …

Genome-Scale Mining of Novel Anchor Proteins of Corynebacterium glutamicum

K Lin, N Zhao, Y Cai, Y Lin, S Han… - Frontiers in Microbiology, 2022 - frontiersin.org
The display of recombinant proteins on the surfaces of bacteria is a research topic with many
possible biotechnology applications—among which, the choice of host cell and anchoring …

Extensive Reannotation of the Genome of the Model Streptomycete Streptomyces lividans TK24 Based on Transcriptome and Proteome Information

J Droste, C Rückert, J Kalinowski, MB Hamed… - Frontiers in …, 2021 - frontiersin.org
Streptomyces lividans TK24 is a relevant Gram-positive soil inhabiting bacterium and one of
the model organisms of the genus Streptomyces. It is known for its potential to produce …