Transpeptidases are powerful tools for protein engineering but are largely restricted to
acting at protein backbone termini. Alternative enzymatic approaches for internal protein …

Asparaginyl ligases have been extensively utilized as valuable tools for site-specific
bioconjugation or surface-modification. However, the application is hindered by the …

In recent years, a few asparaginyl endopeptidases (AEPs) from certain higher plants have
been identified as efficient peptide ligases with wide applications in protein labeling and …

Peptide asparaginyl ligases (PALs) are precision tools for peptide cyclization, cell‐surface
labelling, protein semisynthesis and protein conjugation. PALs are expressed as inactive …

Through amide bond formation, peptide ligases (such as sortase A and subtiligase)
introduce specific protein modifications, providing a specific and affordable tool for protein …