Diversity and evolution of class 2 CRISPR–Cas systems
Class 2 CRISPR–Cas systems are characterized by effector modules that consist of a single
multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the …
multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the …
CRISPR-Cas: adapting to change
BACKGROUND The arms race between prokaryotes and their perpetually evolving
predators has fueled the evolution of a defense arsenal. The so-called CRISPR-Cas …
predators has fueled the evolution of a defense arsenal. The so-called CRISPR-Cas …
The CRISPR spacer space is dominated by sequences from species-specific mobilomes
SA Shmakov, V Sitnik, KS Makarova, YI Wolf… - MBio, 2017 - Am Soc Microbiol
Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein
(CRISPR-Cas) systems store the memory of past encounters with foreign DNA in unique …
(CRISPR-Cas) systems store the memory of past encounters with foreign DNA in unique …
CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids
R Pinilla-Redondo, J Russel… - Nucleic acids …, 2022 - academic.oup.com
Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile
genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components …
genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components …
Next-generation precision antimicrobials: towards personalized treatment of infectious diseases
Highlights•Precision antimicrobials represent a novel approach for treating infectious
diseases.•The role of the microbiota in health and disease is indisputable and so is the need …
diseases.•The role of the microbiota in health and disease is indisputable and so is the need …
Spacer capture and integration by a type IF Cas1–Cas2-3 CRISPR adaptation complex
RD Fagerlund, ME Wilkinson… - Proceedings of the …, 2017 - National Acad Sciences
CRISPR-Cas adaptive immune systems capture DNA fragments from invading
bacteriophages and plasmids and integrate them as spacers into bacterial CRISPR arrays …
bacteriophages and plasmids and integrate them as spacers into bacterial CRISPR arrays …
Atypical organizations and epistatic interactions of CRISPRs and cas clusters in genomes and their mobile genetic elements
Prokaryotes use CRISPR–Cas systems for adaptive immunity, but the reasons for the
frequent existence of multiple CRISPRs and cas clusters remain poorly understood. Here …
frequent existence of multiple CRISPRs and cas clusters remain poorly understood. Here …
Genome-wide correlation analysis suggests different roles of CRISPR-Cas systems in the acquisition of antibiotic resistance genes in diverse species
S Shehreen, T Chyou, PC Fineran… - … Transactions of the …, 2019 - royalsocietypublishing.org
CRISPR-Cas systems are widespread in bacterial and archaeal genomes, and in their
canonical role in phage defence they confer a fitness advantage. However, CRISPR-Cas …
canonical role in phage defence they confer a fitness advantage. However, CRISPR-Cas …
Beyond the canonical strategies of horizontal gene transfer in prokaryotes
Highlights•The reported diversity of HGT strategies in prokaryotes has recently
increased.•HGT could require MGE or be MGE-independent.•Some MGE only mobilize their …
increased.•HGT could require MGE or be MGE-independent.•Some MGE only mobilize their …
Bioinformatic evidence of widespread priming in type I and II CRISPR-Cas systems
ABSTRACT CRISPR-Cas systems provide bacteria and archaea with adaptive immunity
against invading genetic elements, such as plasmids, bacteriophages and archaeal viruses …
against invading genetic elements, such as plasmids, bacteriophages and archaeal viruses …