L-asparaginase, an amidohydrolase that catalyses break down of L-asparagine into aspartic
acid and ammonia, is one of the most prominent biopharmaceuticals. L-asparaginase (LA) …

Over the past few years, there has been a surge in the industrial production of recombinant
enzymes from microorganisms due to their catalytic characteristics being highly efficient …

Microorganisms from extreme environments, such as the Antarctic ecosystems, have a great
potential to produce enzymes with novel characteristics. Within this context, L-asparaginase …

Abstract L-asparaginase (L-ASNase) is a life-saving medication used in the treatment of
Acute Lymphoblastic Leukemia (ALL) which affects over 60,000 people yearly. However, L …

Genetic engineering for heterologous expression has advanced in recent years. Model
systems such as Escherichia coli, Bacillus subtilis and Pichia pastoris are often used as host …

Heterologous expression of L-asparaginase (L-ASNase) has become an important area of
research due to its clinical and food industry applications. This review provides a …

Aim: To review the available literature about heterologous expression of fungal L-
asparaginase (L-ASNase). Materials & methods: A search was conducted across PubMed …

L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia
and aspartic acid. This enzyme has applications in the food industry and health sector …

HSA-GCSF m fusion protein production by recombinant Pichia. pastoris with traditional DO-
stat or methanol ON-OFF feeding strategy often suffers problems such as a limited methanol …

Thermostability is an important and desired feature of therapeutic proteins and is critical for
the success or failure of protein drugs development. It can be increased by PEGylation …