3DFlex: determining structure and motion of flexible proteins from cryo-EM
Modeling flexible macromolecules is one of the foremost challenges in single-particle
cryogenic-electron microscopy (cryo-EM), with the potential to illuminate fundamental …
cryogenic-electron microscopy (cryo-EM), with the potential to illuminate fundamental …
Conformational heterogeneity and probability distributions from single-particle cryo-electron microscopy
Single-particle cryo-electron microscopy (cryo-EM) is a technique that takes projection
images of biomolecules frozen at cryogenic temperatures. A major advantage of this …
images of biomolecules frozen at cryogenic temperatures. A major advantage of this …
Methods for cryo-EM single particle reconstruction of macromolecules having continuous heterogeneity
B Toader, FJ Sigworth, RR Lederman - Journal of molecular biology, 2023 - Elsevier
Macromolecules change their shape (conformation) in the process of carrying out their
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …
functions. The imaging by cryo-electron microscopy of rapidly-frozen, individual copies of …
3D variability analysis: Resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM
Single particle cryo-EM excels in determining static structures of protein molecules, but
existing 3D reconstruction methods have been ineffective in modelling flexible proteins. We …
existing 3D reconstruction methods have been ineffective in modelling flexible proteins. We …
3D flexible refinement: structure and motion of flexible proteins from Cryo-EM
Single particle cryo-EM excels in determining static structures of biological macromolecules
such as proteins. However, many proteins are dynamic, with their motion inherently linked to …
such as proteins. However, many proteins are dynamic, with their motion inherently linked to …
Frozen in time: analyzing molecular dynamics with time-resolved cryo-EM
SJ Amann, D Keihsler, T Bodrug, NG Brown… - Structure, 2023 - cell.com
Molecular machines, such as polymerases, ribosomes, or proteasomes, fulfill complex tasks
requiring the thermal energy of their environment. They achieve this by restricting random …
requiring the thermal energy of their environment. They achieve this by restricting random …
Emerging themes in CryoEM─ Single particle analysis image processing
Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …
Better, faster, cheaper: recent advances in cryo–electron microscopy
Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for
visualizing biological objects, which has been driven by advances across the entire pipeline …
visualizing biological objects, which has been driven by advances across the entire pipeline …
Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures
Using a new consensus-based image-processing approach together with principal
component analysis, the flexibility and conformational dynamics of the SARS-CoV-2 spike in …
component analysis, the flexibility and conformational dynamics of the SARS-CoV-2 spike in …
Integrating molecular models into CryoEM heterogeneity analysis using scalable high-resolution deep Gaussian mixture models
Resolving the structural variability of proteins is often key to understanding the structure–
function relationship of those macromolecular machines. Single particle analysis using …
function relationship of those macromolecular machines. Single particle analysis using …